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- What is true about 'cracking' of emulsion? Select one: O a. The primary emulsion does not become white due to coalescence of the dispersed phase O b. The globules of dispersed phase rise to the surface O c. Cracking is reversible and redispersion can be achieved by shaking O d. It is a result of phase inversion due to prolong storage2 L of 5 mg/mL gelatin solution from 1 g/mL gelatin solutionScenario You have a polypeptide chain that is linked by disulfide bond. If beta-mercaptoethanol is applied, there is no disulfide bond anymore. What has happened to the polypeptide chain after the detergent abovehas been applied. Does it increase, decrease or remain the same?
- What does the absorbance at 280 nm detect? aromatic amino acid side chains chaotropic salts EDTA contamination purines and pyrimidinesUnder what pH conditions can a protein not bind to the beads in a column? pH = -pKa pH = pI pH = 7 pH = pKa In size exclusion/gel filtration chromatography, the elution order is dependent upon Molecular weight Concentration Overall Charge Enzymatic Activityhow many ml of sterile water injection must be mixed with two 4 ml vials of sterile hypertonic NaCl 7% USP to make the mixture isotonic? phar
- Why is agarose an ideal gel matrix for electrophoresis experiments? Because it forms a liquid at all temperatures. Because it is un-ionized and uncharged Because it is positively charged fs f6 f7 fg fg f1o $ [8 19 4 6 7 8. 4. 5 R Y F G J 2 K エWhich of the following is the densest TAG? O trimyristin O triolein tristearin tripaltristearin mitin100ml of LB media with 25 μg/ml of Amp and 100 μg/ml of Kan final concentration. You have 100ml of LB provided and Amp and Kan stocks at 100 mg/ml and 50 mg/ml provided. Determine how much of each antibiotic stock solution you need to add to 100ml of LB to reach desired antibiotic concentration.
- List the five components of isoelectric focusing gel list the five components of isoelectric focusing gelPut the following steps for running a gel in order. first Remove comb and put gel int v [ Choose ] Load samples into gel Let gel solidify for 15-20 minutes Connect electrodes and turn on power to run gel second Pour heated, liquid agarose into gel cartridge Remove comb and put gel into bottom of electrophoresis box Pour buffer into electrophoresis box to cover gel third fourth Pour heated, liquid agarose in v fifth Load samples into gel sixth Connect electrodes and turn vList the five components of an isoelectric focussing gel