o What is a pure culture? o Describe the procedure for making a streak plate from a clinical specimen (like a urine culture). o What is the desired outcome (result) of a streak plate? o Clinically, why is it important to make a pure culture from a clinical specimen? What do you do next in order to treat the patient?

Which of the following is a reason to run simultaneous tests on known positive and negative controls when identifying an unknown microbe using biochemical tests? O The controls maintain the differential qualities of the test medium The controls will ensure that the unknown microbe multiplies on the medium O The controls can show if there was contamination of the tubes with another species O The controls can show if there was a recent mutation in your unknown microbe MacBook Air DII F12 80 888 F7 F5 F4 F3 * & %2$ %23 6 7 8. 4 5

A 7 year old female patient is admitted to a local hospital for a greenstick radius break.  The break is repaired, the patient is sent home and receives a cast a few days later.  The patient arrives back at the hospital 20 days afterwards complaining of pain, sweeling under the cast.  Upon removal of the cast, there is notable swelling, pus, and a furuncle. a.  what type of specimen should you collect? b. What type of instant test would you perform? c. if your clinic ran out of the instant tests, what lower cost method would you use to identify the species? d. What would be the typical course of treatment? e. Due to some cases of antibiotic resistant infections at the same hospital, the doctors want to use a specimen and test for antibiotic resistence, What test would you perform, and which antibiotics should the doctor avoid prescribing?

In this experiment, different concentrations of sucrose are first prepared. Then, cylinders of potato (Solanum tuberosum) tuber are made using a cork borer, which are then trimmed to remove the skin and be of the same length. The cylinders are blot dried and weighed prior to being placed in each of the test tubes containing the sucrose solution. After 20 minutes, the cylinders were removed from the solution, blot dried, then weighed again. Study the results of the experiment shown in Table 1. Table 1. Changes in the weights of potato tuber tissue immersed in various concentrations of sucrose for 20 minutes Concentration (M) 0.0 0.2 0.4 0.6 0.8 1.0 Initial weight 3.76 3.15 3.29 3.82 3.40 3.52 Final weight 4.44 3.40 3.09 3.32 2.71 2.57 Change in weight (g) 0.68 0.25 -0.20 -0.50 -0.69 -0.95 % Change in weight 18.09 7.94 -6.08 -13.09 -20.29 -26.99 Calculate the water potential of the potato tuber cells. Express the answer in both bars and megapascal

After placing sample in this instrument, what are the names of two fractions in the tube (choose all that are correct)? OPellet O Base O Supernatant O Fluid O Precipitation

To identify a positive control in your lab report, should you list any test tube with positive results? Why or why not? What is a negative control? Which substance is a negative control for each of the tests in this lab exercise?