wavelength absorption for the amino acids below from shortest wavelength to longest in the UV region.

Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
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Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
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UV-vis spectroscopy can be used to determine protein and nucleic acid concentrations, provided the user knows
the molar extinction coefficient of the protein or nucleic acid. Rank the wavelength absorption for the amino
acids below from shortest wavelength to longest in the UV region. 

The image depicts the chemical structures of four amino acids in their zwitterionic forms. Each amino acid structure includes an amino group, a carboxyl group, and a distinctive side chain which defines the specific amino acid.

1. **First Structure**: 
   - **Amino Acid**: Isoleucine
   - **Details**: This structure features a branched alkyl side chain with a chiral center, composed of a methyl and ethyl group bonded to the main carbon chain.

2. **Second Structure**:
   - **Amino Acid**: Tryptophan
   - **Details**: The side chain includes an indole group, consisting of a bicyclic structure with a benzene ring fused to a five-membered nitrogen-containing pyrrole ring.

3. **Third Structure**:
   - **Amino Acid**: Phenylalanine
   - **Details**: This amino acid contains a benzyl side chain, characterized by a benzene ring connected to the main carbon chain.

4. **Fourth Structure**:
   - **Amino Acid**: Histidine
   - **Details**: The side chain features an imidazole ring, a five-membered ring containing two nitrogen atoms, which can participate in various chemical reactions due to its heterocyclic nature.

In all four structures, the amino group is protonated and the carboxyl group is deprotonated, forming the zwitterionic state typical at physiological pH.
Transcribed Image Text:The image depicts the chemical structures of four amino acids in their zwitterionic forms. Each amino acid structure includes an amino group, a carboxyl group, and a distinctive side chain which defines the specific amino acid. 1. **First Structure**: - **Amino Acid**: Isoleucine - **Details**: This structure features a branched alkyl side chain with a chiral center, composed of a methyl and ethyl group bonded to the main carbon chain. 2. **Second Structure**: - **Amino Acid**: Tryptophan - **Details**: The side chain includes an indole group, consisting of a bicyclic structure with a benzene ring fused to a five-membered nitrogen-containing pyrrole ring. 3. **Third Structure**: - **Amino Acid**: Phenylalanine - **Details**: This amino acid contains a benzyl side chain, characterized by a benzene ring connected to the main carbon chain. 4. **Fourth Structure**: - **Amino Acid**: Histidine - **Details**: The side chain features an imidazole ring, a five-membered ring containing two nitrogen atoms, which can participate in various chemical reactions due to its heterocyclic nature. In all four structures, the amino group is protonated and the carboxyl group is deprotonated, forming the zwitterionic state typical at physiological pH.
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