Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN: 9780134580999
Author: Elaine N. Marieb, Katja N. Hoehn
Publisher: PEARSON
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- Explain the basis for identification using biochemical testing. – Again, discuss enzymatic pathways, how do we make enzymes in a cell, what is the blueprint for an enzyme? Please keep this answer to 2-3 sentences
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- SDS-PAGE reagents that play a role in denaturing the protein sample include (Select all that applies) Bromophenol blue APS Sodium Dodecyl Sulfate Acetic Acid Glycerol Heat Beta-Mercaptoethanol TEMEDarrow_forwardCase Study: Case Study: Catalase Activity Catalase H,O, 0 + O2 (2) Catalase is an enzyme that converts hydrogen peroxide (H,O) to oxygen and water. An experiment investigated the effect of temperature on the rate of the catalase reaction Small (10 cm) test tubes were used for the reactions, each containing 0.5 cm of enzyme and 4 cm of hydrogen peroxide. Reaction rates were assessed at four temperatures (10°C, 20°C, 30'C. and 60 C). For each temperature, there were two reaction tubes (e.g. tubes 1 and 2 were both kept at 10°C). The height of oxygen bubbles present after one minute of 10°C 20°C 30°C 60°C Height of охудen bubbles 4 cm H,02+ 0.5 cm erzyme reaction was used as a measure of the reaction rate: a laster reaction rate produced more bubbles. The entire experiment, involving eight tubes, was repeated on two separate days. Tubes Tubes Tubes 1 & 2 Tubes 3 & 4 5 & 6 7 & 8 1. What is the purpose of this experiment? inrestrgatee effect of demperalure on the rate ot he 2. Write a…arrow_forwardCan I have help with number 2 and 3? 2) 2What is the specific function of the enzyme catalase? 3) Catalase is an enzyme found in aerobic cells. What does this mean?arrow_forward
- Determine the minimum inhibitory concentrations (MIC) for compounds 1, 2, and 3 based on the microdilution results.arrow_forwardIf the higher value of KM resulting in the new plot ( red curb ) is due to the presence of an enzyme inhibitor is inhibitor reversible or irreversible? And why?arrow_forwardExplain the difference between native vs denatured PAGE electrophoresis of proteins with respect to status of protein and the reagents used, as well as applications.arrow_forward
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