Q22: The results of kinetic experiments of an unidentified enzyme were used to create a plot of the initial reaction velocity (Vo) versusthe substrate concentration ([S]).V, (μmol-min-¹)0.351/₂0.30.250.20.150.10.050050 100 150 200 250 300 350 400 450The enzyme kinetics data were also used to create a double-reciprocal plot of121086[S] (nM)4-0.01 -0.005 0 0.005 0.01 0.015 0.02 0.0251/[S]Does it appear that the enzyme follows Michaelis-Menten kinetics?versus1[S] Does it appear that the enzyme follows Michaelis-Menten kinetics?yesVmaxnocannot be determinedNow use the double-reciprocal plot to determine the maximum velocity, Vmax, and the Michaelis constant, KM, of the enzyme.=KM =μmol-minnM

Michaelis Menten kinetics and Lineweaver Burk plot show the activity of the enzyme catalyzed…

Answered: V, (μmol-min-¹) 0.35 1/V₂ 0.3 0.25 0.2…

Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

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V, (μmol-min-¹) 0.35 1/V₂ 0.3 0.25 0.2 0.15 0.1 0.05 0 0 50 100 150 200 250 300 350 400 450 [S] (nm) The enzyme kinetics data were also used to create a double-reciprocal plot of - versus Vo [S] 12 10 8 6