Use your textbook or a reliable online resource to help you define the following 4 terms in your lab notebook: substrate (reactant), enzyme, active site, energy of activation (EA)

2 W 1 Z MacBook Air PRE-LAB ASSIGNMENT Enzyme - Functional Analysis Instructions: Read the Introduction section of the lab and watch the following video: https://www.youtube.com/watch?v=V6wHXtO9kIA. Then complete the questions below. 1. Define Enzymes. What type of macromolecules are enzymes? 2. What are two advantages of having enzyme-catalyzed chemical reactions in living cells? reaction? 3. What is a substrate? Where on an enzyme does the substrate specifically bind to during a chemical in each of following: 4. When an enzymatic reaction is in progress, do you expect to see an increase, decrease or no change (i) substrate (ii) product (iii) enzyme 5. How can you measure enzyme activity? 77

Which of the following terms describes only the protein component of a typical enzyme? cosubstrate prosthetic group apoenzyme holoenzyme cofactor

Enzymes are biological catalysts that enhance the rate of a reaction by: stabilizing the transition state. decreasing the amount of free energy released. increasing the activation energy. increasing the amount of free energy released. O increasing the energy in the transition state.

Classify each phrase as describing an enzyme, an active site, or a substrate. Enzyme may be a phosphate group a protein containing an active site a substance that the enzyme acts upon a biological catalyst Active site Answer Bank Substrate a specific enzyme location that binds with the substrate

The site on an enzyme that will bind the substrate is called the active site. activation site. prosthetic group. catalyst. metastable site.

le.com/document/d/1aV35WZHe72GRC05NgDa6otm70ZIPIBC-T2NiT7IwmOU/edit Chemical Reactions ☆ 回 G Tools Add-ons Help Last edit was 2 minutes ago E - E E I text Georgia 12 BIUA 7 3 4 5 I 6 The table below lists enzymes that function in different locations in the human body, and the temperature and pH ranges of these locations. Different enzymes are secreted in each of the three locations. Enzyme Location Temperature (°C) pH ptyalin mouth 36.7-37.0 6.5-7.0 pepsin stomach 37.3-37.6 1.0-3.0 trypsin small intestine 37.3-37.6 7.5-9.0 • Using the pH column in the chart, explain why pepsin can work in the stomach, but not the small intestines. State how a fever of 40°C would most likely affect the activity of these enzymes and support your answer using information from the temperature column in the chart. 27°F

Select all statements that are true about enzymes. 1.They are always proteins 2.They form a temporary intermediate compound with a substrate.  3.They all function optimally at 37C 4.Enzymes have different properties 5.They can be denatured if the pH of their environment is too high

What does the last number in the numeric designation of enzymes refer to? Group of answer choices A serial number that frequently indicates the order in which the enzyme was recognized. The major enzyme class A subclass which further characterize the major class some information about the substrate

A biologist investigating enzyme function plotted the activity of a particular enzyme (y-axis) vs pH (x-axis).  Experiment A was performed at 30 oC, experiment B at 10 oC and experiment C at 100 oC.  Experiments A, B, and C all used the same type of enzyme, they simple tested the enzyme's activity at different temperatures.  Using your knowledge of enzyme activity, answer the questions based on the results shown in the graph. (Answer question B) Graph included B.  Why is there no hill in the middle of line C?