The purpose of this experiment is to determine how the activity of an enzyme can change under certain conditions: temperature, pH, substrate concentration Introduction Purpose statement is specific, complete and uses proper scientific vocabulary Hypothesis statement is clear, logical and scientifically sound Contains sufficient background information relevant to the lab Accurate use of examples and scientific terminology Clear, concise (max 1 page; single spaced) Step 1: Obtain a sample amount of a hard fruit or vegetable, such as an apple, cut it into smaller equal pieces, and measure on a digital scale of 10g. Step 2: Get 100ml of room temperature tap water and add it into a glass cup, heat it on a hot plate until reaches 37 Celsius Step 3: Add the apple sample into the glass cup with the tap water and mix gently. Let the mixture to sit for 5 minutes. Step 4: With a kitchen thermometer measure the temperature of the apple mixture. Step 5: In a glass cup add 3mL of 3% hydrogen peroxide, also add 0.5g of baking soda into the glass with the 3% hydrogen peroxide. Step 6: Add 1 mL of the apple mixture into the glass with baking soda and 3% hydrogen peroxide, start a stopwatch. Step 7: Record the time until the reaction occurs, also using a rate of scale from 0 - 5 estimate. Step 8: Repeat steps 4 to 7 twice more for the total of 3 times Step 9: Repeat steps 4 to 9 for each of the conditions below: High pH - Add 0.5 mL of vinegar toe the apple mixture, repeat steps from 4 to 9 Low pH - Add 0.5 mL of baking soda to the apple mixture, repeat steps 4 to 9 High Temperature - Heat the apple mixture to 50 Celsius, repeat steps 4 to 9 Low Temperature - Cool the apple mixture to 10 Celsius, repeat steps 4 to 9 Substrate concentration: Use 2 mL & 4 mL of the apple mixture instead of the 1 mL which was used in step 2 - 3.
Proteins
We generally tend to think of proteins only from a dietary lens, as a component of what we eat. However, they are among the most important and abundant organic macromolecules in the human body, with diverse structures and functions. Every cell contains thousands and thousands of proteins, each with specific functions. Some help in the formation of cellular membrane or walls, some help the cell to move, others act as messages or signals and flow seamlessly from one cell to another, carrying information.
Protein Expression
The method by which living organisms synthesize proteins and further modify and regulate them is called protein expression. Protein expression plays a significant role in several types of research and is highly utilized in molecular biology, biochemistry, and protein research laboratories.
The purpose of this experiment is to determine how the activity of an enzyme can change under certain conditions: temperature, pH, substrate concentration
Introduction Purpose statement is specific, complete and uses proper scientific vocabulary Hypothesis statement is clear, logical and scientifically sound Contains sufficient background information relevant to the lab Accurate use of examples and scientific terminology Clear, concise (max 1 page; single spaced) Step 1: Obtain a sample amount of a hard fruit or vegetable, such as an apple, cut it into smaller equal pieces, and measure on a digital scale of 10g. Step 2: Get 100ml of room temperature tap water and add it into a glass cup, heat it on a hot plate until reaches 37 Celsius Step 3: Add the apple sample into the glass cup with the tap water and mix gently. Let the mixture to sit for 5 minutes. Step 4: With a kitchen thermometer measure the temperature of the apple mixture. Step 5: In a glass cup add 3mL of 3% hydrogen peroxide, also add 0.5g of baking soda into the glass with the 3% hydrogen peroxide. Step 6: Add 1 mL of the apple mixture into the glass with baking soda and 3% hydrogen peroxide, start a stopwatch. Step 7: Record the time until the reaction occurs, also using a rate of scale from 0 - 5 estimate. Step 8: Repeat steps 4 to 7 twice more for the total of 3 times Step 9: Repeat steps 4 to 9 for each of the conditions below: High pH - Add 0.5 mL of vinegar toe the apple mixture, repeat steps from 4 to 9 Low pH - Add 0.5 mL of baking soda to the apple mixture, repeat steps 4 to 9 High Temperature - Heat the apple mixture to 50 Celsius, repeat steps 4 to 9 Low Temperature - Cool the apple mixture to 10 Celsius, repeat steps 4 to 9 Substrate concentration: Use 2 mL & 4 mL of the apple mixture instead of the 1 mL which was used in step 2 - 3.
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