Use correct sig figs The concentration of a purified monoclonal antibody was measured using UV280 nm. The sample was diluted (200 μL of purified antibody in 800 μL buffer) prior to analysis using a spectrophotometer. Calculate the concentration of antibody in the purified fraction if the Abs=0.95 of the diluted antibody. The molar absorptivity is known to be 191,411.6 M-1cm-1 and the molecular weight is 150 kDa. The pathlength for the cuvette is 1 cm.

With an A260 value of 1.12 and a DNA concentration of 56.0 ng/mL, what is the pathlength of the optical system used within the Nanodrop?

Please explain.

2 if there is a stock solution of 2000 (ug/ml) , is it possible to use SERIAL DILUTION to generate the following 8 tubes with their concentrations? A 2000 (ug/ml) B 1500 (ug/ml) C 1000 (ug/ml) D 750  (ug/ml) E 500  (ug/ml) F 250  (ug/ml) G 125  (ug/ml) H 0 (ug/ml)

Please dont provide handwrittin solution.../.

(b) Both laboratories used 10 micrograms of protein each in their kinetic assays. Protein concentrations weredetermined by the Bradford protein assay. Assay conditions employed in the two labs (pH, temperature,etc.) were also identical. What would be the most plausible cause for the discrepancy in the Vmax valuesfor the compound I? Explain.Recall that the Bradford assay measures total protein amounts in sample solution based on complexformation between a dye and proteins. Also, the assay solution used in both labs does not contain anyinhibitors.

Match the types of data to the example.                                                                                                         Precise measurements                                                                                                         Assay standard                                                                                                         Positive control                                                                                                         Quantitative results                                                                                                         Negative control                                                                                                         Qualitative results                                                                                                         Standard curve…

With an accompanying brief narrative, write a short lab report (brieflydescribing what you did) and in a results section, show your standard curve andtabulate the concentration of the unknown protein samples provided using thethe Bradford Assay and UV absorbance)

Using the SDS-PAGE and the calibration curve given, calculate the size of the lysozyme and ovalbumin bands from this lysozyme purification. Lysozyme is the bottom band in the samples, ovalbumin is the most intense band in the first three samples, and the first and last lanes are molecular mass standards.

V2