What are the three states of raft assemblies? Describe each.

What is the difference and compare the light source and path of light of a immunofluorescence microscope from a compound light microscope?

Use the image below to answer this question and the three questions that follow:   a) what is the correct description of the hemoglobin for Paul? b) Where is the + pole of the electrophoresis for the gel shown in the previous question? c) Refer to gel illustration above. Which of the following statements  provides the best explanation for the difference in mobility  of HbA and  HbS? 1) HbA  is larger in size than HbS 2) HbA is smaller in size than HbS 3) HbA  has less negative charge than HbS under  electrophoretic conditions used 4) HbA is smaller and more negatively charged under the electrophoretic conditions used 5) HbA  is larger and has a less negative charge under the electrophoretic conditions used, whereas HbS is smaller and has a more negative charge

Which of the following is TRUE about immunofluorescence microscopy where we localize proteins using fluorescently tagged antibodies? Pick the best answer: We can use it to resolve 2 points that are 10 nanometers apart. The emission wavelength of the fluorophore is typically shorter than the excitation wavelength. The resolution can be further improved with increased magnification. All else being equal, a fluorophore that emits red light will give better resolution than a fluorophore that emits green light. Based on what we discussed in class, we can use the technique on live samples therefore enabling us to see the dynamic movement of cytosolic proteins in living cells. None of the above

A patient's antibody screen is resulted as positive. What should be done to test units for transfusion?     Question 7 options:   A)  Antigen type the patient's red cells and crossmatch units positive for the same antigens.   B)  Determine the reacting antibody and crossmatch units negative for the corresponding antigen.   C)  Crossmatch ABO compatible blood at the immediate spin phase.   D)  Determine the reacting antibody and confirm that the donor did not have any similar antibodies.

How would you prepare a 1:350 dilution of secondary antibody in a total volume of 3mL?

Crossmatching donor red cells for a patient involves combining:     Question 2 options:   A)  2 drops of donor red cells with 1 drop of patient plasma   B)  2 drops of patient red cells with 2 drops of patient plasma   C)  1 drop of donor red cells with 2 drops of patient plasma   D)  1 drop of donor red cells with 1 drop of patient plasma

If you were using the ELISA to look for the presence of antibodies and the sample gave a negative result, does this mean that there were no antibodies present? Explain.

a)At what wavelength (nm) on a spectrometer can this color be quantified?                      b) Record your results. Indicate on this page which boxes turned color.                    A B C + - 1:2           1:10           1:100             c) Based on these results, which patient(s) definitely have lupus, which definitely do not, and which require further testing?

The following questions refer to the analysis of cells using flow  cytometry  A) When analyzing cells in a flow cytometer how do you ensure you are analyzing single  cells rather than doublets or clusters of cells and why is that important?  B) How do forward versus side scatter plots enable the characterization of different  types of blood cells in the blood of a patient? How does this relate to a potential  diagnosis of acute leukemia?  C) How do you determine if a patient has B cell versus T cell Leukemia? Why is it  important to know?