Thanks To improve peptide separation by using a HPLC system, trifluoroacetic acid actsas mobile-phase modifier was added during the preparation of mobile phase.The preparation was performed by a postgraduate student as following:"2.851 g trifluoroacetic acid (MW: 114.02 g/mol) was made up to 500 cm³ in agraduated flask. To this solution, 50 cm³ of ethanol was added, and after mixingthe mobile phase was placed in the solvent reservoir and pumping wascommenced at 1.5 cm min-1."Based on the given preparation procedure, identify THREE mistakes that weremade. A chemist attempted to synthesize benzoic acid by the oxidation of benzylalcohol. However, a mixture of products was obtained when chromatographywas used to separate them. Only three organic compounds were obtained whichare benzaldehyde, benzyl alcohol and benzoic acid. In addition, three spectra areshown in Figure 2.Wavenumber cmSpecrum (a)40003000200015001000500100-50-Spectrum (b)4000300020001500100050010050-Spectrum (c) 40003000200015001000500100-50-Figure 2Match the spectra to their respective compounds with justification.Transmitance (%)Transmittance (%)Transmittance (%)

The objective of the question is to find the three mistakes that were made while preparing mobile…

To improve peptide separation by using a HPLC system, trifluoroacetic acid acts as mobile-phase modifier was added during the preparation of mobile phase. The preparation was performed by a postgraduate student as following: "2.851 g trifluoroacetic acid (MW: 114.02 g/mol) was made up to 500 cm³ in a graduated flask. To this solution, 50 cm³ of ethanol was added, and after mixing the mobile phase was placed in the solvent reservoir and pumping was commenced at 1.5 cm³ min¹." Based on the given preparation procedure, identify THREE mistakes that were

To improve peptide separation by using a HPLC system, trifluoroacetic acid acts as mobile-phase modifier was added during the preparation of mobile phase. The preparation was performed by a postgraduate student as following: "2.851 g trifluoroacetic acid (MW: 114.02 g/mol) was made up to 500 cm³ in a graduated flask. To this solution, 50 cm³ of ethanol was added, and after mixing the mobile phase was placed in the solvent reservoir and pumping was commenced at 1.5 cm³ min¹." Based on the given preparation procedure, identify THREE mistakes that were

Chemistry

10th Edition

ISBN:9781305957404

Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste

Publisher:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste

Chapter1: Chemical Foundations

Section: Chapter Questions

Problem 1RQ: Define and explain the differences between the following terms. a. law and theory b. theory and...

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