You want to incorporate an unnatural amino acid (not one of the 20 ‘normal’ amino acids) into a protein of interest. What steps would have to be taken in order to successfully incorporate this into the protein. Assume you already have the ‘Wild type’ gene in a plasmid expression vector.  (Hint: remember the central dogma DNA>mRNA>tRNA-> Protein)

Chemistry for Today: General, Organic, and Biochemistry
9th Edition
ISBN:9781305960060
Author:Spencer L. Seager, Michael R. Slabaugh, Maren S. Hansen
Publisher:Spencer L. Seager, Michael R. Slabaugh, Maren S. Hansen
Chapter21: Nucleic Acids And Protein Synthesis
Section: Chapter Questions
Problem 21.41E: The -chain of hemoglobin is a protein that contains 146 amino acids residues. What minimum number of...
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You want to incorporate an unnatural amino acid (not one of the 20 ‘normal’ amino 
acids) into a protein of interest. What steps would have to be taken in order to 
successfully incorporate this into the protein. Assume you already have the ‘Wild 
type’ gene in a plasmid expression vector.  (Hint: remember the central dogma DNA
>mRNA>tRNA-> Protein)

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