You want to incorporate an unnatural amino acid (not one of the 20 ‘normal’ amino acids) into a protein of interest. What steps would have to be taken in order to successfully incorporate this into the protein. Assume you already have the ‘Wild type’ gene in a plasmid expression vector. (Hint: remember the central dogma DNA>mRNA>tRNA-> Protein)
You want to incorporate an unnatural amino acid (not one of the 20 ‘normal’ amino acids) into a protein of interest. What steps would have to be taken in order to successfully incorporate this into the protein. Assume you already have the ‘Wild type’ gene in a plasmid expression vector. (Hint: remember the central dogma DNA>mRNA>tRNA-> Protein)
Chemistry for Today: General, Organic, and Biochemistry
9th Edition
ISBN:9781305960060
Author:Spencer L. Seager, Michael R. Slabaugh, Maren S. Hansen
Publisher:Spencer L. Seager, Michael R. Slabaugh, Maren S. Hansen
Chapter21: Nucleic Acids And Protein Synthesis
Section: Chapter Questions
Problem 21.41E: The -chain of hemoglobin is a protein that contains 146 amino acids residues. What minimum number of...
Related questions
Question
You want to incorporate an unnatural amino acid (not one of the 20 ‘normal’ amino
acids) into a protein of interest. What steps would have to be taken in order to
successfully incorporate this into the protein. Assume you already have the ‘Wild
type’ gene in a plasmid expression vector. (Hint: remember the central dogma DNA
>mRNA>tRNA-> Protein)
Expert Solution
This question has been solved!
Explore an expertly crafted, step-by-step solution for a thorough understanding of key concepts.
Step by step
Solved in 2 steps
Recommended textbooks for you
Chemistry for Today: General, Organic, and Bioche…
Chemistry
ISBN:
9781305960060
Author:
Spencer L. Seager, Michael R. Slabaugh, Maren S. Hansen
Publisher:
Cengage Learning
Organic And Biological Chemistry
Chemistry
ISBN:
9781305081079
Author:
STOKER, H. Stephen (howard Stephen)
Publisher:
Cengage Learning,
General, Organic, and Biological Chemistry
Chemistry
ISBN:
9781285853918
Author:
H. Stephen Stoker
Publisher:
Cengage Learning
Chemistry for Today: General, Organic, and Bioche…
Chemistry
ISBN:
9781305960060
Author:
Spencer L. Seager, Michael R. Slabaugh, Maren S. Hansen
Publisher:
Cengage Learning
Organic And Biological Chemistry
Chemistry
ISBN:
9781305081079
Author:
STOKER, H. Stephen (howard Stephen)
Publisher:
Cengage Learning,
General, Organic, and Biological Chemistry
Chemistry
ISBN:
9781285853918
Author:
H. Stephen Stoker
Publisher:
Cengage Learning
Introduction to General, Organic and Biochemistry
Chemistry
ISBN:
9781285869759
Author:
Frederick A. Bettelheim, William H. Brown, Mary K. Campbell, Shawn O. Farrell, Omar Torres
Publisher:
Cengage Learning