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Label three sets of eight microfuge tubes (1-8) and add the volumes of BSA stock solution (2 mg/ml) and water to each labeled tube as indicated in Table 3. Be sure to calculate, and enter, the values for column 4 of this table. Table 3 STD Curve Volume BSA Volume Water (µ) Final BSA Concentration tube (mg/ml) Stock (ul) number 100 2 5 95 3 10 90 4 20 80 5 40 60 6 60 40 7 80 20 100 00

Label three sets of eight microfuge tubes (1-8) and add the volumes of BSA stock solution (2 mg/ml) and water to each labeled tube as indicated in Table 3. Be sure to calculate, and enter, the values for column 4 of this table. Table 3 STD Curve Volume BSA Volume Water (µ) Final BSA Concentration tube (mg/ml) Stock (ul) number 100 2 5 95 3 10 90 4 20 80 5 40 60 6 60 40 7 80 20 100 00

Human Anatomy & Physiology (11th Edition)
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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**Preparing Standard Curve for BSA Concentration Measurement** To create a standard curve for BSA (Bovine Serum Albumin) concentration measurement, you will need to label three sets of eight microfuge tubes numbered from 1 to 8. Add specific volumes of a BSA stock solution (2 mg/ml) and water to each labeled tube as indicated in Table 3. Subsequently, calculate and enter the final BSA concentration values for column 4 of this table. **Table 3: Preparation of BSA Standard Curve** | STD Curve Tube Number | Volume BSA Stock (µl) | Volume Water (µl) | Final BSA Concentration (mg/ml) | |-----------------------|-----------------------|-------------------|---------------------------------| | 1 | 0 | 100 | | | 2 | 5 | 95 | | | 3 | 10 | 90 | | | 4 | 20 | 80 | | | 5 | 40 | 60 | | | 6 | 60 | 40 | | | 7 | 80 | 20 | | | 8 | 100 | 0 | | ### Explanation of Table 3 The table consists of four columns: 1. **STD Curve Tube Number:** This column lists the tube numbers from 1 to 8. 2. **Volume BSA Stock (µl):** The volume of BSA stock solution (2 mg/ml) to be added to each tube. This volume increases incrementally from 0 to 100 µl. 3. **Volume Water (µl):** The volume of water to be added to each tube, which decreases correspondingly as the volume of BSA stock solution increases, maintaining a total volume of 100 µl per tube. 4. **Final BSA Concentration (mg/ml):** This column is for recording the final BSA concentration in each tube after mixing the BSA stock solution and water. The final BSA concentration for each tube can be calculated using the formula: \[ \text{Final BSA Concentration (mg/ml)} = \left( \frac{\text{Volume BSA Stock (µl)} \times \text{BSA Stock Concentration (mg/ml)}}{\text{Total Volume
Transcribed Image Text:**Preparing Standard Curve for BSA Concentration Measurement** To create a standard curve for BSA (Bovine Serum Albumin) concentration measurement, you will need to label three sets of eight microfuge tubes numbered from 1 to 8. Add specific volumes of a BSA stock solution (2 mg/ml) and water to each labeled tube as indicated in Table 3. Subsequently, calculate and enter the final BSA concentration values for column 4 of this table. **Table 3: Preparation of BSA Standard Curve** | STD Curve Tube Number | Volume BSA Stock (µl) | Volume Water (µl) | Final BSA Concentration (mg/ml) | |-----------------------|-----------------------|-------------------|---------------------------------| | 1 | 0 | 100 | | | 2 | 5 | 95 | | | 3 | 10 | 90 | | | 4 | 20 | 80 | | | 5 | 40 | 60 | | | 6 | 60 | 40 | | | 7 | 80 | 20 | | | 8 | 100 | 0 | | ### Explanation of Table 3 The table consists of four columns: 1. **STD Curve Tube Number:** This column lists the tube numbers from 1 to 8. 2. **Volume BSA Stock (µl):** The volume of BSA stock solution (2 mg/ml) to be added to each tube. This volume increases incrementally from 0 to 100 µl. 3. **Volume Water (µl):** The volume of water to be added to each tube, which decreases correspondingly as the volume of BSA stock solution increases, maintaining a total volume of 100 µl per tube. 4. **Final BSA Concentration (mg/ml):** This column is for recording the final BSA concentration in each tube after mixing the BSA stock solution and water. The final BSA concentration for each tube can be calculated using the formula: \[ \text{Final BSA Concentration (mg/ml)} = \left( \frac{\text{Volume BSA Stock (µl)} \times \text{BSA Stock Concentration (mg/ml)}}{\text{Total Volume
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