Answered: CITRATE UTILIZATION TEST WAS PERFORMED…

CITRATE UTILIZATION TEST WAS PERFORMED IN A CULTURE MEDIA WHICH CONTAIN A STRONG BUFFER. AND EVEN A SURE POSITIVE ORGANISM GET A NEGATIVE RESULT. WHY?

Complete Textbook Of Phlebotomy

5th Edition

ISBN:9781337464314

Author:Hoeltke

Publisher:Hoeltke

Chapter10: Sample Preparation And Handling

Section: Chapter Questions

Problem 4CT

See similar textbooks

Related questions

Q: If molecules are expected to move from high concentration to low concentration, why didn’t all the…

A: Dialysis tubing is a kind of semi-permeable membrane which is made up of cellulose acetate. It is…

Q: In starch test describe and explain the change color in results: A. Yellow to Brown B. Blue to Black

A: The starch test is used to detect the presence of starch in the solution where few drops of iodine…

Q: My question is: Could you explain steps? (etc. why do we heat or add chemicals like nacl or ammonium…

A: Casein is the major protein component found in milk. It is generally a phosphoprotein which is…

Q: You do a serial dilution on an actively growing bacterial culture broth. Doing a plate count of your…

A: A serial dilution is the stepwise dilution of a substance in solution. It is done to find out the…

Q: What type of molecule is shown here? Give its biological importance? ÇH2OH но OH test question 3.png…

A: It is the structure of glucose molecule. It is present in pyran ring form. So it is called gluco…

Q: Explain why a green metallic sheen is formed when E. coli is grown on Eosin Methylene Blue Agar.

A: Escherichia coli is the gram negative bacteria.

Q: Why should the use of nichrome or iron containing loop/wire be avoided as an inoculating device when…

A: The oxidase is a test used in microbiology to identify bacteria that produce cytochrome c oxidase.…

Q: Which of the following would be best for sterilizing a pre-sealed case of plastic medical supplies?

A: Answer - Autoclave

Q: Organism 1 Organism 2

Q: What are the advantages of the API 20E test compared to regular biochemical tube media

A: API 20E test helps in the determination of bacteria from lab-scale activated sludge treatment…

Q: The most efficient disinfectant chlorine, discuss why? ( subject: medical microbiology lab )

A: Step 1 Disinfectants are chemical agents that are applied to abiotic objects to destroy or…

Q: Need help Can a pure culture be prepared from a mixed culture in a broth or plate? Explain.

A: INTRODUCTION How to make a pure culture from mixed culture is explained below.

Q: You requested the following media/reagents for your unknown. What are you testing for with each…

A: The majority of bacteriological culture media are available commercially as pre-made or as bases…

Q: Pretend the results below were obtained for water from your well. The 10 mL tubes are Full Strength,…

A: Most Probable Number or MPN method is a way to estimate the number of microorganisms in the water…

Q: A powdered complex medium can be stored for months in the lab. However, after rehydrating the medium…

Q: The chart below represents the results (absorbance readings) of your Bradford Assay. Use the data…

A: The Bradford protein assay is a quick and accurate spectroscopic analytical procedure used to…

Q: True or False: The turbidity of a sample incubated in a liquid medium can be assessed to estimate…

A: Turbidity means cloudiness present in a sample.

Q: Explain why TSIA is a suitable medium for identifying enteric bacteria. To do this you will have to…

A: Enteric bacteria are those bacteria which are present in intestines and can be either gut flora or…

Q: What is the EFFECT and RATIONAL of Molten agar mixed with bacterial suspension has temperature…

A: Bacteria can be cultured in either a liquid media or a solid media based on the purpose of culturing…

Q: These were the treatments from the catalase concentration experiment. Which tube would show the most…

A: Enzymes are proteins that catalyze the reaction so that the activation energy required by the…

Q: The aerotolerance test is used to determine the oxygen requirements for bacterial growth.

A: FALSE.

Q: how many bacteria/ml of the diluted broth would be present on average

A: 37 bacteria/ml of the diluted broth would be present on average.

Q: Suppose your professor handed you a bottle of kombucha with 2.0 ml in it and told you to make a…

Q: heat the samples. Would increasing the concentration of acid in the solution, in lieu of heating,…

A: In Barfoed's test heating is used to break the bonds between the disaccharides forming…

Q: The directions of Mannitol Salt agar was “suspend 28.9 grams in 1000 ml distilled water”. You will…

A: Mannitol salt agar is of importance in medical diagnostic use. It contains up to 10% of sodium…

Q: Joshua is showing a bacterium having high pH requirement for its growth. With this, he has to…

A: For titration low conc. of Acid and base used because at the time of titration, we add volume. if…

Q: the process of converting TAG to fatty acid salt and alcohol.* Please choose one correct answer only…

A: Triglycerides or Triacylglycerol (TAG) are lipids comprised of three fatty acids esterified with…

Q: What is the purpose of the vial that does not contain chara? (the solution with chara turned…

A: bromothymol blue is an acid base indicator. It is blue if the solution is alkaline and it become…

Q: give one reason why is it necessary to keep stirring the agar medium mixture in a beaker when…

A: Agar is a heteropolysaccharide, which is composed of different carbohydrate units. Agar is obtained…

Q: Why does the oxidase test must be performed from blood agar or another medium without a fermentable…

A: Biochemical tests are used to identify microbial diversity based on variations in the biochemical…

Q: You are testing a sample of water from a swimming pool. You count a plate made from a 1 mL transfer…

A: Dilution is a process by which solution (water or diluents) are added to the solvent (main solution)…

Q: You perform a nitrate test. After adding solutions A and B, the broth remains colorless. Why should…

A: Nitrate test helps us to determine the production of nitrate reductase enzyme. By the activity of…

Q: Medium used for cellulase production contained (g/L): NaNO3, 3; MgSO4 7H2O, 0.5; K2HPO4, 1; KCl, 0.5…

A: Culture media is proper nutrient for the growth of the microorganisms, which help to grow a variety…

Q: You are working in a hemodialysis clinic and have just received a shipment of dialysate solution.…

A: Answer: DIALYSATE SOLUTION = This is the solution of pure water , electrolytes , and salts used in…

Q: SEE EXAMPLE IN THE IMAGE A mixture of 0.20M acetic acid and 0.30M sodium acetate is given. Calculate…

A: The Henderson Hasselbalch equation for calculation of the pH of a solution of weak acid and its…

Q: Salt Treatment Tap Water Treatment Distilled Water Treatment Which of these experimental…

A: A hypertonic solution contains a higher concentration of solutes compared to another solution.…

Q: If a bacteria is inoculated onto milk agar and an area of clearing is observed around the bacterial…

A: Milk agar or skim milk agar is used to differentiate and cultivate microbes based on their…

Q: Starch Hydrolysis: In many tests it is acceptable to read a positive result before the inoculation…

A: Starch hydrolysis test is used to identify a bacterial species. In this test, test bacteria are…

Q: A culture medium consisting of known amounts of glucose, NaCl, and ammonium and potassium phosphate…

A: Culture medium is a nutrient medium which is used to grow microorganisms by providing appropriate…

Q: Growth medium: You are running different experiments on microbes and need to make sure you choose…

A: A growth medium is a solid/ liquid media designed to support the growth of a population of…

Q: You are asked to make a 1:1000 dilution of a bacterial culture in a final volume of 5000 mL. What…

A: In this question, we are given with a solution of 1X concentration. We have to determine volume of…

Q: Many culture media produce colorful solutions due to the presence of which are water soluble organic…

A: Colony color or pigmentation is mostly exploited characteristics of bacteria which aids in the…

Q: Which of the following items is the least suitable for sterilization by autoclave? a. Water for…

A: An autoclave is a machine that is used to perform industrial and scientific processes that require…

Q: Agar is a solidifying agent used in media preparation. How and why does the agar concentration in…

Q: What are two advantages of filtration in microbial control? Explain how the filtration process…

A: According to the question, we have to describe two advantages of filtration in microbial control. In…

Q: True or false: Ethidium bromide is added to your hot liquid agar solution to help sepa bands by size…

A: Agarose gel electrophoresis is one of the major techniques that is used in molecular biology…

Q: Superoxide ion is toxic to strict anaerobes because they lacka. catalase b. peroxidase c. dismutase…

A: A superoxide basically is a compound that do contain the superoxide ion, having the chemical…

Q: An unknown bacterium produces colorless colonies when inoculated onto an EMB plate. Predict what you…

A: EMB stands for Eosin Methylene Blue. It is a selective stain used for the growth of gram-negative…

Concept explainers

Microbial Growth

The development of microbial growth is influenced by various natural conditions (both physical and synthetic) like pH, temperature, saltiness, gas focus (O2, CO2, and H2), nutrients, water action, and assimilation. Knowing the distinctive physical and chemical factors that influence the development and endurance of microbial growth in the climate will assist with reinforcing endeavors outfitted towards their control. These lines lessen or diminish their harmful impacts on humankind, creatures, and the climate.

Question

CITRATE UTILIZATION TEST WAS PERFORMED IN A CULTURE MEDIA WHICH CONTAIN A STRONG BUFFER. AND EVEN A SURE POSITIVE ORGANISM GET A NEGATIVE RESULT. WHY?

Expert Solution

This question has been solved!

Explore an expertly crafted, step-by-step solution for a thorough understanding of key concepts.

SEE SOLUTION Check out a sample Q&A here

Step 1 Citrate Utilization test

VIEW

Step 2 ANSWER

VIEW

Step by step

Solved in 2 steps

SEE SOLUTION Check out a sample Q&A here

Knowledge Booster

Learn more about

Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, biology and related others by exploring similar questions and additional content below.

Similar questions

You aseptically transfer 1 ml of your optional liquid culture into 99 ml of sterile water. What is the dilution factor. Show work
The agar turns pink because the enzyme produces blank which causes a ph change to blank?
You applied hydrogen peroxide to a bacterial culture growing on a Petri dish and obtained bubbles within 10 seconds of application. What test did you perform? Catalase O Oxidase Triple Sugar Iron agar O MacConkey agar
In your opinion and based on your knowledge, what is the most important component that will be tested using the reagent test strips?
Which ingredient is included in the motility test to make the results easier to see? bromthymol blue ferric citrate TTC phenol red
During media preparation, you observed that the agar medium did not solidify after sterilization even after incubating it for 1 hour at room temperature. What could be the reason behind this?
Helicobacter pylori, a bacterium that causes stomach ulcers, can grow in medium with a pH above 5. It can survive but not grow in pH 2 medium as long as the medium contains Urea. You inoculate a pH 2 broth with 1,000,000 H. pylori and for 8 hours the organism did not grow. The next morning however, the medium has become quite turbid, suggesting that the organism has grown. Explain how this could happen. What phylum and class does this organism belong?
Suspend 28.0 grams in the 1000 mL distilled water. Heat to boiling to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (1210C) for 15 minutes. Cool to 45-500C. If desired the medium can be enriched with 5-10% blood or other biological fluids. Mix well and pour into sterile Petri dishes. 1. Determine the amount of dehydrated medium needed to prepare 50 nutrient agar plates. Include amount for 2 additional plates as excess to compensate for compounding losses.
You are asked to add 2 mL of bacterial culture to 98 mL of water. What is your dilution factor? Show solution
Design a serial dilution procedure to achieve a 56-colony count, from a sample with 8.75x105 CFU/mL bacterial concentration. show solution
Agar is a solidifying agent used in media preparation.a. What is its origin? b. What makes it ideal for cultivation of microbes? c. How and why does the agar concentration in semisolid media differ from conventional solid media?
An unknown bacterium produces colorless colonies when inoculated onto an EMB plate. Predict what you would see if you inoculated that same unknown onto the Mannitol salt agar media?