Three human genomic DNA fragments (A, B, and C) are sequenced and revealed the following: fragment A contains gene 2 and 3; fragment B contains gene 2 and 4; fragment C contains gene 1 and 4; Based on this, what should the order of the three DNA fragments in a sequencing contig?

Approximately how many Okazaki fragments are synthesized in thereplication of the human genome?

How many Illumina clusters would you need to generate 15X coverage of the human genome, assuming your read lenght is 600 bp? (assume a human genome size of 3 billion bp) - 2.5 billion - 75 million - 7 million - 100 million

1. a) What is a microsatellite sequence? b) The sequence 5' ...GTACATAAGACACACTAAGTAGCTTA...3' contains a microsatellite. Provide the microsatellite information using the proper nomenclature (i.e., with brackets and subscript).

In the practical you have been analysing a human genomic library. You know from your calculations that only a small proportion of the human genome is represented, even when the entire class results are considered. Therefore, the chance of finding a particular single-copy gene in your library is very small. Outline a strategy for constructing a genomic DNA library more representative of the entire human genome. You will need to consider alternative vectors and the efficiency of transformation of the bacterial cells.