This is a walk-through of Minimum Inhibitory Concentration, MIC: This tray has 8 rows, 4 for Staphylococcus aureus (SA) and 4 for Methicillin-Resistant S. aureus (MRSA). Each row has 12 compartments, or wells. The wells have different concentrations (ug/ml) of the antibiotic Oxacillin in them. From Left to Right, the concentration of Oxacillin is going acillin concentration, µg/ml 2 4 64 32 16 8 1 0.5 0.25 0.13 0.06 0 SA down up The same amount of S. aureus broth culture is added to each well and the tray is incubated. A color change shows that S. MRSA aureus grew in a given well. What is the lowest concentration at which SA is inhibited (does not grow)? 64mg/ml What is the lowest concentration at which MRSA is inhibited? What is the MIC for Oxacillin vs SA? What is the MIC for Oxacillin vs MRSA?
This is a walk-through of Minimum Inhibitory Concentration, MIC: This tray has 8 rows, 4 for Staphylococcus aureus (SA) and 4 for Methicillin-Resistant S. aureus (MRSA). Each row has 12 compartments, or wells. The wells have different concentrations (ug/ml) of the antibiotic Oxacillin in them. From Left to Right, the concentration of Oxacillin is going acillin concentration, µg/ml 2 4 64 32 16 8 1 0.5 0.25 0.13 0.06 0 SA down up The same amount of S. aureus broth culture is added to each well and the tray is incubated. A color change shows that S. MRSA aureus grew in a given well. What is the lowest concentration at which SA is inhibited (does not grow)? 64mg/ml What is the lowest concentration at which MRSA is inhibited? What is the MIC for Oxacillin vs SA? What is the MIC for Oxacillin vs MRSA?
Chapter10: Reconstitution Of Powdered Drugs
Section: Chapter Questions
Problem 9.4P
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Transcribed Image Text:This is a walk-through of Minimum Inhibitory
Concentration, MIC:
This tray has 8 rows, 4 for Staphylococcus aureus (SA) and 4 for
Methicillin-Resistant S. aureus (MRSA). Each row has 12
compartments, or wells. The wells have different
concentrations (ug/ml) of the antibiotic Oxacillin in them. From
Left to Right, the concentration of Oxacillin is going
down
acillin concentration, pg/ml
64 32 16 8
4
2
1 0.5 0.25 0.13 0.06 0
SA
dn
The same amount of S. aureus broth culture is added to each
well and the tray is incubated. A color change shows that S.
aureus grew in a given well.
MRSA
What is the lowest concentration at which SA is inhibited (does
not grow)?
64mg/ml
What is the lowest concentration at which MRSA is inhibited?
What is the MIC for Oxacillin vs SA?
What is the MIC for Oxacillin vs MRSA?
- Notes
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