Given this research scenarios kindly construct a research framework. A researcher is interested to test the antibacterial activity of a Philippine Plant crude extracts against Beta-lactamase producing Klebsiella pneumonia isolated in a hospital setting. He will use the following concentrations of crude extract, 500mg/ml, 250mg/ml and 100mg/ml. Positive control: Tetracycline 10mg/ml and DMSO as the negative control.
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Given this research scenarios kindly construct a research framework.
- A researcher is interested to test the antibacterial activity of a Philippine Plant crude extracts against Beta-lactamase producing Klebsiella pneumonia isolated in a hospital setting. He will use the following concentrations of crude extract, 500mg/ml, 250mg/ml and 100mg/ml. Positive control: Tetracycline 10mg/ml and DMSO as the negative control.
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- essential oils have been studied as potential antimicrobial agents. These naturally occuring compounds are linked to having the following type of activity in clinical trials: virucidal, septicidal, bactricidal, fungicidalWith the aid of diagrams, illustrate the biochemical basis of the following: Allopurinol used in the management of gout Hydroxyurea as an antineoplastic agent 6-mercaptopurine, an anticancer agent Trimethoprim, an antibacterial agentIf you have 4 compounds and you test them for antimicrobial activity using a disk diffusion assay and one is very active, one is moderately active, one is slightly active and one is not active at all, draw the disk, the zone of inhibition that you would see and a pretend numerical value to indicate the size of the zone in mm, give each one a number 1 – 4 and rank them from most effective to least effective as an antimicrobial.
- Given the data, discuss the following results of a hypothetical Biochemical test performed on 4 bacterial isolates .Given this, if you used 6g of vitamin Z powder to make 20 ml of solution, what is the % concentration of this solution? (I gave the image since I don't know if that info is needed to solve this question.)It also gives a follow-up, if you can help here too: You work in a lab as a summer student. One of your tasks is to make sure that there is enough cell culture medium containing antibiotics to grow bacteria. One day you realize that there is only 5 ml of 10% Antibiotic stock solution in the freezer. You decide to use it all to prepare the working culture medium with 0.01% antibiotic. In the lab there is plenty of growth medium without antibiotics. (Note: dilution in medium is like dilution in water). You remember the equation to make dilutions of stock solutions. You usually use this formula to calculate the required volume of a stock solution, but you realize it can apply here as well, even though the unknown is the final volume. So, you make that dilution. Given that each bacterial…Briefly comment on the use of alkaline phosphatase assays as a diagnostic aid for a single named common medical condition.
- As a public health engieer you have been tasked wiyh designing a plug flow reactor for the disinfection of Giardia lamblia using either free chlorine and monochloramine. The objective is a three log reduction in the concentration of Giardia lamblia. The kinetics of chlorine decay can be described by a first order coefficient of 0.1 d-1. It is also given that the appropriate contact time, t10, equals the hydrualic residence time (HRT or td) of the plug flow reactor. Question: At a temperature of 5oC and a pH of 7.0, what is the volume of the PFR if the flowrate of the water to be disinfected is 1000 L/d for chlorine and monochloramine Disinfection data in the table below can be used.Example of a Protein Purification Scheme: Purification of the Enzyme Xanthine Dehydrogenase from a Fungus Volume Total Total Specific Percent Fraction (mL) Protein (mg) Activity Activity Recovery 1. Crude extract 2. Salt precipitate 3. Ion-exchange chromatography |4. Molecular-sieve chromatography 5. Immunoaffinity chromatography 3,800 22,800 2,460 0.108 100 165 2,800 1,190 0.425 48 65 100 720 7.2 29 40 14.5 23 1.8 275 152.108 11 Calculate the specific activity of step#4. Note that percent recovery=% Yield.Sydney Brenner isolated Salmonella typhimurium mutants that were implicated in the biosynthesis of tryptophan and would not grow on minimal medium. When these bacterial mutants were tested on minimal medium to which one of four compounds (indole glycerol phosphate, indole, anthranilic acid, and tryptophan) had been added, the growth responses shown in the following table were obtained. Mutant Minimal medium Anthranilic acid Indole glycerol phosphate Indole Tryptophan trp-1 − − − − + trp-2 − − + + + trp-3 − − − + + trp-4 − − + + + trp-6 − − − − + trp-7 − − − − + trp-8 − + − − + trp-9 − − − − + trp-10 − − − − + trp-11 − − − − + Give the order of indole glycerol phosphate, indole, anthranilic acid, and tryptophan in a biochemical pathway leading to the synthesis of tryptophan. Indicate which step in the pathway is affected by each of the mutations.
- Nitrate reduction test can be used to differentiate between Pseudomonads and Enterobacteriaceae. You inoculated your Nitrate broth with your unknown; after incubation, you added 5 drops of reagent A and 5 drops of reagent B. You observe a red color within 2 minutes. Is this a + or a - reaction? Assuming you did not get a red color, you then added a pinch of Zinc and this time tou obtained a red color. What does this mean?Thermal images are photographs of the heat emittedby an object. Researchers have used thermal imaging ofplants to isolate mutants that overproduce abscisic acid.Suggest a reason why they are warmer than wild-typeplants under conditions that are normally nonstressful.After testing the potency of different β-lactamase inhibitors against purified OXA-M290 enzyme, the antimicrobial activity of combinations of β-lactam antibiotics and β-lactamase inhibitors is tested against the E. coli KGH1 strain that produces OXA-M290. The results of these studies can be used to identify the best antibiotic to treat an infection caused by this E. coli strain. Broth microdilution tests are carried out with a panel of β-lactam antibiotics and β-lactamase inhibitors to determine their MICs against E. coli KGH1. These tests are carried out in a 96-well microplate. Three β-lactam antibiotics (amoxicillin, ceftazidime, and ertapenem) and three β-lactamase inhibitors (sulbactam, tazobactam, and vaborbactam) are tested. The rows in the microplate correspond to the following antibiotics and inhibitors: Row A: amoxicillinRow B: amoxicillin + sulbactamRow C: ceftazidimeRow D: ceftazidime + sulbactamRow E: ertapenemRow F: ertapenem + sulbactamRow G: ertapenem + tazobactamRow H:…