Using the table above, make the following unit conversions. Determine th point the appropriate number of places. 1. 25 µL =, mL 8. 8500 µL = L 2. 3 L = µL 9. 64 TL = GL 3. 150 cL = dL 10. 120000 nL = µL 4. 5000 pL = nL 5. 75 L = kL 6. 800 nL =. cL
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- Choose the one answer that fits best. Which of the following statements regarding the proper procedure for using Micropipettes is NOT correct? O a. You cannot use a 2-20 µl micropipette to pipet 200 µl O b. To expel all the liquid from the tip, you have to press the eject button O c. To draw up solution, press and hold the plunger at the first stop before entering the solution O d. Micropipettes always require the use of a disposable plastic tip O e. While pipetting, micropipettes should always be held as straight as possibleIn the spread plate method, why is the volume plated usually limited to not more than 0.1 mL?A cell count was performed on a serous fluid specimen. As the fluid was cloudy, a 1:5 dilution was prepared. The counts were: 34 and 32; 5 squares on each side were counted. What is the average cell count per L? Side 1: 34 x 1 x (1/(5x0.1)) x 5 = 340 per µl Side 2: 32 x 1 x (1/(5x0.1)) x 5= 320 per µl
- How much of 10,000x SYBR safe would you add to 50 ml to make a final concentration of 1x? V1= 0.005mL B.How will you set up the serial dilution? How many tubes do you need? What is the concentration in each? How much LB will you add to each tube? What volume of cells will you add?Using 25 μL of serum, how much diluent would be needed to create a 1:2 dilution? ______ μLTo the right is an image of a dilution that was performed. The volume above the top arrow indicates the volume that should be transferred to the next tube (i.e. Tube 1). The volume listed at the bottom-right of tube 1 indicates the volume of diluent that should be added to that tube. The stock concentration is 50μg/ml and you want to make a solution in tube 1 with a concentration of 0.4μg/ml and a total volume of 3ml. Stock ?ml Tube 1 ?ml a. How many milliliters of stock solution needs to be added to Tube 1? Round your answer to three decimal places (e.g. 0.111). b. How many milliliters of diluent needs to be added to Tube 1? Round your answer to three decimal places.
- If 0.1 ml is added to 99.9 ml medium, this results in a dilution of: Select one: а. 10-3 b. 10-4 С. 10-1 d. 10-5 e. 10-2Using 10 μL of a patient's serum, how much diluent would be needed to create a 1:10 dilution? ______ μLWhat is the minimal concentration (MIC) for disinfectant number 1? Just write the corresponding number including the decimal as written. ug/mL. Disinfectant 1 2 3 4 concentration 0.1 ug/mL +++ ++++ ++ +++ 0.2 ug/mL ++ ++ + ++ 0.5 ug/mL + + 1.0 ug/mL 5.0 ug/mL subculture 1 2 3 4 0.1 ug/mL ++++ ++++ ++++ ++++ 0.2 ug/mL ++++ ++++ ++++ ++++ 0.5 ug/mL ++++ ++++ ++++ 1.0 ug/mL ++++ 5.0 ug/mL Submit response
- 1mL Stock #1 1mL 9mL #2 9mL #3 1mL A 9mL #4 0.1mL 1mL O O B 9mL #5 1mL 1mL 9mL #6 O Using the picture serial dilution scheme and the following information (plate A has 276 colonies, plate B has 298, plate C has 2, and plate D has 30), calculate the average number of colony forming units per mL in the stock tube. Make sure to only use countable plates. Round your answer to the nearest one. Write only the number with any needed commas or decimals. Do not include units. 0.1mL 1mk D OI do not understand the results of this experiment, explain what the results of this experiment are and the purpose Table 2 Absorbance vs CoCl2 concentration Test Tube Number Cobalt Chloride Concentration (mol/mL) Absorbance at 510 nm 1 0.000 0.000 2 0.009 0.024 3 0.018 0.055 4 0.027 0.085 5 6 0.036 0.045 0.112 0.138 7 (Unknown) 0.088Below are the results of our disc diffusion study using antiseptics and disinfectants. Use the results to answer the following questions: 1:10 Lab diluted Lab Tincture bleach Disinfectant Isopropanol soap Lysol of iodine BL DIS ISP SO LYS T of I E.coli 40 mm 20 mm 10mm 5mm 15mm 60 mm Bacillus 50 mm 35 mm 15 mm 8mm 20mm 70 mm cereus chemical(s) best at inhibiting both Gram- negative and Gram-positive bacteria [ Choose ] chemical least effective at inhibiting both [ Choose ] bacteria