What wavelength is most suitable for quantitative analyses for an analyte exhibiting the UV-Vis Spectrum shown below (which has an interfering analyte with the spectrum shown in adashed line)? Explain why?1.0rABF0.75-C0.50E0.25350400450500550600650Wavelength, nmFigure 3-19 Choice of experimental wavelength. Solid line: measured species.Dashed line: interfering species. See text for delails.Absorbance

Quantitative analysis is the method of determination of concentration of an analyte where teh amount…

Answered: What wavelength is most suitable for…

Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

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Make a standard curve by using the values given in the table and determine the concentration of unknown sample? Tube 0,5 µg/ µl BSA 0,15 M NaCl Comassi Brillant Blue Solution Absorbance 595 nm 1 0 100 µl 900 µl 0 2 10 µl 90 µl 900 µl 0,105 3 20 µl 80 µl 900 µl 0,233 4 30 µl 70 µl 900 µl 0,41 5 40 µl 60 µl 900 µl 0,455 6 50 µl 50 µl 900 µl 0,589 Unknown Sample 10 µl 90 µl 900 µl 1,2
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