the most efficient general strategy for whole genome sequencing is ? (a) double the coding sequence after sequencing the proteins (b) shotgun sequence and assemble based on overlaps (c) identify mutations that affect glycolysis (d) obtain recombinant DNA clone maps before starting the sequencing (e) obtain comprehensive SNP maps before determining the order of DNA clone

Q: You need to amplify a gene from a source DNA for cloning in any cloning vector, which enzymes will…

A: The method of DNA cloning is used to produce the multiple identical copies of the specific template…

Q: The following diagram shows one-half of a restriction site. (a) Draw the other half. GAC G I C (b)…

A: Introduction: The genetic material in the living organism that transfer from one generation to…

Q: Kary Mullis invented the Polymerase Chain Reaction (PCR) technique in 1983 which won him the 1993…

A: Polymerase Chain Reaction: A very small DNA sample can be amplified (or part of it amplified) to a…

Q: Restriction endonucleases (RE) are one of the most used enzymes in biotechnology for recombinant DNA…

A: Restriction enzymes are the enzymes which cut DNA sequences at a particular site. Restriction enzyme…

Q: Although many cloning applications involve introducing recombinant DNA into bacterial host cells,…

A: Cloning can be defined as the process by which identical copies of the organisms are created. More…

Q: a. What sequence information about a gene is lackingin a cDNA library?b. Can clones in a cDNA…

A: The cDNA or complementary DNA is derived from mRNA which is transcribed from genomic DNA (gDNA).  It…

Q: Polymerase Chain Reaction (PCR) was invented by Kary Mullis in 1983. This technique had indeed…

A: The annealing temperature is the temperature at which the primers bind to the denatured DNA strands.…

Q: ndom mutations anywhere within the genome in living cells (in vivo)           b) random…

A: In research to create mutations it is very necessary to first decipher the type of organism in which…

Q: and they/ it start(s) from the mRNA 4-Some/ one application(s) for DNA are/ is Therefore, the…

A: Single-stranded RNAs of the type known as messenger RNA, or mRNA, are used to make proteins. mRNA is…

Q: The soil bacterium Agrobacterium tumefaciens, also regarded as 'nature's own genetic engineer', is…

A: As per our guidelines, we are supposed to answer only three sub-parts. Kindly repost the question…

Q: 18- Protein sequences can be more informative than DNA sequences. Which of the following is NOT one…

A: DNA sequences and protein sequences are both important sources of information for understanding the…

Q: High-throughput DNA sequencing technology ... 1.) is far cheaper than Sanger sequencing on a…

A: The term "DNA sequencing" refers to a common laboratory procedure for finding out the precise…

Q: Experiment: Transformation of the bacterium Streptococcus pneumoniae. Based on the study, four…

A: Introduction The genetic modification of a cell caused by the direct uptake and integration of…

Q: Which technique rapidly replicates specific DNA fragments without cloning in cells? (a) gel…

A: DNA is the genetic material in most living organisms. It is the information hub of the cell that…

Q: Amplified target regions of four different samples were separated using gel electrophoresis.  DNA…

A: Gel electrophoresis is a lab technique for separating DNA, RNA, and protein mixture based on their…

Q: (1) What is the major difference between the conventional PCR and real time PCR (qPCR) in terms of…

A: Question. What is the major difference between the conventional PCR and real time PCR (qPCR) in…

Q: . The position of the gene for the protein actin in the haploid fungus Neurospora is known from the…

A: DNA is the genetic material in most living organisms. It is the information hub of the cell that…

Q: (A) After three cycles of PCR, how many DNA molecules are present that correspond precisely to the…

A: PCR stands for the polymerase chain reaction, which is used to generate larger copies of the gene of…

Q: Predict whether loss of the following E. coli genes would be lethal or not: (a) dnaB (which encodes…

A: Escherichia coli (E.coli) consists of many essential genes that are required for the survival of the…

Q: Polymerase Chain Reaction (PCR) was invented by Kary Mullis in 1983. This techniqu had indeed…

A: PCR is used to amplify a specific DNA sample.

Q: The transposase enzyme is essential for transposition because

A: Answer:  Transposase is an enzyme which required for the transposition of elements of DNA.

Q: 3). You mutagenize bacteria, plate them to the permissive temperature, then replica plate to the…

A: At the permissive temperature, the mutants in dnaB would likely exhibit a normal phenotype since…

Q: RNAi is a method to assess........ gene expression    gene sequence  gene function  Chromosomal…

A: A gene is a functioning heredity unit made up of DNA, which provides instructions for the creation…

Q: Bacteriophage lambda is as one of the routinely used molecular cloning vectors, which alsoserved as…

A: Gene cloning is a process in which our gene of interest is located into the gene and copied (cloned)…

Q: please solve the  following: (a)Explain how a mutation effects a genotype. (b) Explain how a…

A: The genotype refers to the hereditary material passed among generation, and the phenotype is…

Q: Choose the false statement: O Using the experimental design from Griffith's experiments on rough and…

A: The false statement is option b)- The term 'gene' was coined by Griffith to explain what was being…

Q: Which lane in the gel the gel electrophoresis experiment correspond to reaction of the circular…

A: Restriction endonucleases are specific enzymes that are responsible for cutting the phosphodiester…

Q: Define `transcriptome'.  Briefly describe the genome tiling array technology to `visualize'…

A: Hi dear, here's your answer what you want.can you please give me a like for this answer. * Every one…

Q: Check all the statements that are TRUE regarding 16S or ITS (microbiome) illumina (NGS) sequencing…

A: The Illumina sequencing chemistry is extremely precise, with the great majority of bases scoring Q30…

Q: Can you help with 1a please HELPFUL INFORMATION: When performing classical Sanger or "dideoxy"…

A: Sanger sequencing is done to identify the sequence of DNA. In this process, a particular ddNTP is…

Q: The authors investigate if the switch from the polymerase to the exonuclease site involves releasing…

A: During DNA replication, accuracy is determined by rate of nucleotide incorporation by DNA…

Q: GEMC

A: The full form of GEAC is Genetic Engineering Approval Committee. The Government of India has formed…

Q: r it 6- Complete the following: a) Two fragments of DNA can be joined by a b) Restriction enzymes…

A: Recombinant DNA technology is the process by which we can isolate our gene of interest and then…

Q: . The DNA polymerases are positioned over the following DNA segment (which is part of a much larger…

A: Introduction  DNA replication is very crucial process for the continuation of life as every new…

Q: How many unique primers are required to amplify a single DNA target region?   a) 1 b) 2 c) 4 d)…

A: Targeted sequencing is a fast and low-cost method to detect known and novel variants in specific…

Q: You would expect to find a sequence corresponding to the 5' untranslated region (5'-UTR) of a gene…

A: 5' UTR region of a gene is the untranslated region which is not translated into the amino acids in…

Q: Now you have the gene sequence. Now you would like to clone it into an expression vector to grow up…

A: Answer. The majority of eukaryotic genes contain non-coding DNA. The genes tend to have a split…

Q: The exponential nature of PCR allows spectacular increases in the abundance of a DNA sequence being…

A: DNA is the chemical name for the molecule that carries genetic instructions in all living things.

Q: Fill in the gaps? One major goal/motivation in the development of synthetic genomes is ...... .…

A: In some ways, synthetic biology is similar to genome editing as both involve changes in the genetic…

Q: Mutagenesis is a technique in which genetic information of an organism is altered in a stable manner…

A: There are a few important points : Mutation: A sudden and heritable change in the sequence of the…

Q: The exponential nature of PCR allows spectacular increases in the abundanceof a DNA sequence being…

A: Polymerase chain reaction, or PCR, is a technique to make many copies of a specific DNA region in…

Genome annotation

The technique of linking biological information to genome sequences is termed genome annotation. Gene annotation is the method of identifying gene locations and coding sections. It helps us understand what these genes are doing in the body through establishing structural characteristics and linking them to the actions of various proteins.