the most efficient general strategy for whole genome sequencing is ? (a) double the coding sequence after sequencing the proteins (b) shotgun sequence and assemble based on overlaps (c) identify mutations that affect glycolysis (d) obtain recombinant DNA clone maps before starting the sequencing (e) obtain comprehensive SNP maps before determining the order of DNA clone

a. What is the purpose of molecular cloning?b. What purpose do selectable markers serve in vectors?c. What is the purpose of the origin of replication in aplasmid vector?d. Why do cloning vectors have polylinkers?

1. Discuss two naturally DNA repair pathways, homologous recombination and non-homolgous end-joining, and how each is utlized in mammalian genome manipulation. 2. Describe and explain how the work of Jinek et al contributed to the adaptation of the naturally occuring CRISPR mechanism to function in genome editing, with reference to the experiment(s) involved. 3. Discuss the advantages of gene silencing compared with genome editing, and briefly describe the two main approaches of gene silencing.

recombinant human insulin, produced by bacteria carrying a cloned insulin gene, is now the major form of insulin used to treat diabetes. It is know that the human insulin gene encodes an mRNA which is only 333 nucleotides long, but the entire gene spreads more than 4000 nucleotides. There are 3 exons and 2 introns. 1. What technique can you use inorder to get a functional insulin coding sequence cloned into bacteria and how does this technique work? 2. The technique used in 1, you would need to start with cells cells from the pancreas, why are these the only cells that would work ?

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Q4) What are the 3 main components required for cloning? Q5) What is 16S rDNA, and why would cloning it be useful? Q6) How big is the 16S rDNA ‘amplicon’ we would be cloning?

In order to clone your female pet cat, which of the following individual component is an absolute requirement (means you MUST use the component and you do not have an option not to use it)? O (B) An enucleated oocyte (egg) from your pet cat O (A) A somatic cell nucleus from your pet cat O (C) An enucleated oocyte (egg) from a different cat O (D) Use your pet cat as surrogate mother for the cloned embryo. Both A and B * Previous Quiz sa Next >