The hypoxia-inducible factor (HIF) plays an important role in cellular respiration in response to low O2 levels.   Using molecular biology, it’s possible to generate animal cells that lack HIF. What would occur if these cells were placed in a low O2 environment?   A.  Inhibition of pyruvate decarboxylase complex activity.   B.  Increase rate of respiratory electron transport.    C.  Build-up of acetyl-CoA in mitochondria.

Rotenone is an insecticide that is not toxic to plants, but is highly toxic to fish and insects. (it will also kill mammals, but it doesn’t easily get into our cells). When Rotenone is added to cells in vitro, ATP production stops, oxygen is no longer reduced and NADH builds up. Based on these results, explain what is a possible mechanism of action for Rotenone?

Mature human red blood cells do not have any mitochondria, yet they live for weeks.Predict which respiration processes red blood cells most likely use. List the metabolicproducts you would expect to find in red blood cells that would support your prediction.

You are growing human muscles cells in the lab and you are trying to test how wellthe cells survive with decreasing concentrations of oxygen. As part of yourexperiment, you are measuring the rate of glucose use, the rate of mitochondrial ATPproduction and the pH of the solution surrounding the cells. As the concentration ofoxygen becomes lower änd lower, what changes might you expect to observe inthese cells?A. The rate of glucose use would increase, the rate of mitochondrial ATP productionwould decrease, and the pH of the solution would decrease (become more acidic).B. The rate of glucose use would decrease, the rate of mitochondrial ATP productionwould decrease, and the pH of the solution would increase (become more basic).C. The rate of glucose use would increase, the rate of mitochondrial ATP productionwould increase, and the pH of the solution would decrease (become more acidic)D. The rate if glucose use would decrease, the rate of mitochondrial ATP productionwould decrease, and…