The holoenzyme consists of 2 DNA polymerase core enzymes a single sliding clamp-loader complex two sliding clamp

To completely unfold a globular protein, use both  A denaturant and reducing agent  b smooth ER and secretase  c kinase and ATP d  Restriction endonuclease and DNA ligase e Proteasome and chaperonin

When you are loading the DNA ladder into your gel the volume needed is 5uL. The appropriate micropipette for this volume is a: 1)p2 2)p200 3)p1000 4)p20

Because the polymerization of nucleotides is an endergonic process, energy is required for it to occur. What does DNA polymerase use as its energy source? Group of answer choices ATP that is broken into ADP and P and released Energy from photons of light Entropy created from protein digestion Nucleotides incorporated into a growing chain provide their own energy in the form of trinucleotides

The following image is of an agarose gel. If DNA samples were loaded to this gel and the electrophoresis experiment was started, explain what would happen and why.

Draw a diagram showing what pGEM will look like after it has been digested with BamHI. Be sure to show both strands (with enzyme sequence) and include the origin of replication, the ampicillin resistance gene, 3’ and 5’, and the DNA sequence at the restriction site. It will probably be easiest to simply draw it as an open circle, not a line. Now show what pGEM will look like after it has been digested with EcoRI instead of BamHI. Again, be sure to show both strands and include the origin of replication, the ampicillin resistance gene, 3’ and 5’, and the DNA sequence at the restriction site.

Which one of the following statements about restriction endonucleases is true? all restriction endonucleases join DNA segments at specific sites all restriction endonucleases join DNA at random sites all restriction endonucleases cut DNA at specific sites all restriction endonucleases cut DNA at random sites

A piece of DNA is cut into four fragments as shown below. A solution containing the four fragments is placed in a single well at the top of an agarose gel. Using the information given below, draw (below the well) how you think the fragments will be aligned on the gell following electrophoresis. Label each fragment with its corresponding letter. Each band on the gel will be the same width, equal to the width of the well at the top of the gell. These should be in one lane.  Do smaller or larger (pb) fragments migrate the furthest? Why?

What is the enzyme used to create DNA complementary to mature mRNA? Group of answer choices ligase topoisomerase DNA polymerase reverse transcriptase RNA polymerase transposase

Select the correct order of steps in a polymerase chain reaction. create reaction mixture, denaturation, extension, primer annealing denaturation, create reaction mixture, primer annealing, extension create reaction mixture, denaturation, primer annealing, extension denaturation, create reaction mixture, extension, primer annealing