RNAi is a method to assess........ gene expression    gene sequence  gene function  Chromosomal location of a gene

8) Transposons have two methods of transposition. Briefly describe each one and indicate whether genome is the same size or has increased in size after a transposition event.

Both question correlate with each other

After sequencing a new genome for the first time, functional protein-coding genes are initially identified by:     BLAST searches that look for viral integration in the host genome     Bioinformatic searches that reveal consensus sequences in expressed genes     Aligning expressed exons sequenced from cDNA using transcriptomics     B and C     A and B Many regions of non-coding eukaryotic DNA previously thought to be "junk" are now known to contain sequence elements important to regulating gene expression. What approach can be used to identify important non-coding regulatory regions when annotating a newly sequenced genome?     comparing cDNA to genomic DNA to validate that the gene is expressed     identifying restriction enzyme recognition sequences in the genome     phylogenetic footprinting to identify conserved non-coding sequences     searching for start/stop codons and splice recognition sites that predict where a gene might be located…

What is a genome and what is it composed of? What is thecentral dogma of molecular biology?

You are analyzing cells that express a fusion gene encoding a FLAG-tagged protein of interest. Upon amino acid sequence analysis, you find that the FLAG protein sequence aligns to the sequence at the end of the fusion protein sequence you have. Where is the tag sequence located?   Group of answer choices At the 5’ end of the gene and the C-term of your protein of interest At the 5’ end of the gene and the N-term of your protein of interest At the 3’ end of the gene and the C-term of your protein of interest At the 3’ end of the gene and the N-term of your protein of interest

Identify the word or phrase that is the best match (some will remain blank). (Mouse Genome Informatics, GeneChip array, Confounding variable, 45,000, BLAST, 600, Aceview, BioGPS, SMART, minimize, 17, UCSC Genome Browser, External validity) A. Median number of copies of an mRNA in a cell B. Patterns and sizes of introns and exons C. H3K4me3 locations on chromosomes D. Each treatment given to a different litter of mice E. Find list of knockout mice for a gene D. Find similar proteins F. Random variation G. Tissue expression pattern of a gene H. The extent to which results can be extrapolated

Expression of a gene, in terms of greater production of the protein it encodes, could be increased by ...   Question 12 options: A)  increasing transcription of that gene. B)  inhibiting proteases that break down the protein it encodes C)  increasing the half life of its mRNA transcript D)  A and C E)  A, B and C