The gene encoding the protein/enzyme: a) DNA ligase b) the B clamp c) primase

Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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7. Temperature sensitive mutants have mutations such that the altered protein functions normally at a low or
"permissive" temperature, usually 37 °C or lower. If the temperature is raised to a higher “non-permissive"
temperature, often 40-42 °C, the mutated protein becomes nonfunctional and the organism's phenotype
becomes that of one totally lacking the mutated gene. Predict the outcome of DNA synthesis at the
nonpermissive temperature for each of the proteins below. Predict whether synthesis would stop immediately
or whether a longer time at the non-permissive temperature would be required for complete cessation of DNA
synthesis to occur in the following temperature sensitive mutants. If appropriate predict what the products of
replication would be at the nonpermissive temperature.
The gene encoding the protein/enzyme:
a) DNA ligase
b) the B clamp
c) primase
d) DnaA
Transcribed Image Text:7. Temperature sensitive mutants have mutations such that the altered protein functions normally at a low or "permissive" temperature, usually 37 °C or lower. If the temperature is raised to a higher “non-permissive" temperature, often 40-42 °C, the mutated protein becomes nonfunctional and the organism's phenotype becomes that of one totally lacking the mutated gene. Predict the outcome of DNA synthesis at the nonpermissive temperature for each of the proteins below. Predict whether synthesis would stop immediately or whether a longer time at the non-permissive temperature would be required for complete cessation of DNA synthesis to occur in the following temperature sensitive mutants. If appropriate predict what the products of replication would be at the nonpermissive temperature. The gene encoding the protein/enzyme: a) DNA ligase b) the B clamp c) primase d) DnaA
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