A certain cDNA of size 2 kb hybridized to eight genomicfragments of total size 30 kb and contained two shortESTs. The ESTs were also found in two of the genomicfragments each of size 2 kb. Sketch a possible explanation for these results

Q: The period gene of Drosophila melanogaster encodes for a stretch of Thr-Gly repeated in tandem. In…

A: In this question, it is given that the allele with 20 Thr-Gly repeats produces an amplicon of size…

Q: Describe the banding pattern seen in the gel. What does that tell you about the role of annealing…

A: Gel electrophoresis is a laboratory technique for separating charged molecules such as DNA, RNA, and…

Q: 5. Assume that you are trying to do a gel mobility shift experiment using a normal set of Trp genes.…

A: The gel mobility shift assay is a biochemistry technique used to detect protein complexes with DNA.…

Q: A method for detecting methylated CpGs involvesthe use of a chemical called bisulfite, which…

A: Hi! Since you have posted multiple questions, we are answering only first three sub-parts. Primers…

Q: a. What sequence information about a gene is lackingin a cDNA library?b. Can clones in a cDNA…

A: The cDNA or complementary DNA is derived from mRNA which is transcribed from genomic DNA (gDNA).  It…

Q: If you wanted to express a library of human proteins in yeast, there are several good reasons why it…

A: The objective of the question is to identify the option that is not a valid reason for preferring a…

Q: The human genome 3.4 Gb would be 2.3 meters long if stretched linearly.In not more than 200…

A: Dna is wrapped around nucleosomes.

Q: “DNMT3L Connects Unmethylated Lysine 4 of Histone H3 to de novo Methylation of DNA,” the authors…

A: Figure a and b of the paper “DNMT3L Connects Unmethylated Lysine 4 of Histone H3 to de novo…

Q: The b1 allele encodes a transcription factor that stimulates production of anthocyanin, a purple…

A: It is given that seven tandem repeats were deleted that are located 100,000 bp upstream of the b1…

Q: In yeast cells, telomerase remains active and maintains telomeres of about 300 base pairs. Describe…

A: Telomerase is an enzyme that is made up of a protein complex and an RNA. The RNA contain a conserve…

Q: A series of exonuclease deletions were used to study the promoter of the rice hemA gene, giving the…

A: A promoter can be defined as a DNA sequence where proteins bind and initiate transcription of an…

Q: Sometimes, cDNAs turn out to be “chimeras”; that is, fusions of DNA copies of two different mRNAs…

A: c dna is complementary dna.

Q: Telomeres contain a 3′ overhang region, as shown. Does telomerase require a 3′ overhang to replicate…

A: Telomeres are protein complexes placed at chromosome ends. The specific telomere structure covers…

Q: For insertion elements and simple transposition, what is the function of the inverted repeat…

A: Transposition A transposon also called a jumping gene is the DNA sequence that changes its position…

Q: F ′strains in E. coli are derived from Hfr strains. In some cases, these F ′strains show a high rate…

A: Conjugation is transfer of genes in bacteria.cell to cell contact by F-pilus or sex pilus allows the…

Q: Immortality of Stem cells is supported by the expression of telomerase that can extend the telomere…

A:

Q: What percentage of the DNA sites in yeast are accessible, assuming that the fraction of sites…

A: Yeasts are the microorganism that come under kingdom fungi. Yeast are usually single celled.

Q: You learned in Problem 21 in Chapter 7 that theneurodegenerative disease ALS can be caused by…

A: Amyotrophic lateral sclerosis (ALS) is a paralyzing neurodegenerative disease which is usually fatal…

Q: Although DNA transposons are abundant in the genomes of multicellular eukaryotes, class 1 elements…

A: The transposable elements are sequences which keeps on changing their position in the human genome,…

Q: In Figure 12-4, certain mutations decrease the relativetranscription rate of the β-globin gene.…

A: A promoter is the region of DNA where transcription is initiated. It provides a site for binding of…

Q: E. coli strain BW25113 can grow on (D) Arg as the sole carbon source. A scientist  working with you…

A: Obtain the genomic  from the mice cells that encode for the protease of interest.Fragment the…

Q: Not all inherited traits are determined by nuclear genes (i.e., genes located in the cell nucleus)…

A: The process of producing a genetically identical clone of an animal is called as reproductive…

Q: would the J3480 Code and 59 Modifer Code go on seperate lines under the HCPCS Level II codes column?

A: HCPCS Code: J3480 - Injection, Marcaine, 30 mlModifier: 59 - Distinct procedural service (to…

Q: Budding yeasts such as S. cerevisiae exhibit telomerase activity throughout their life cycles,…

A: DNA is the genetic material in most living organisms. It is the information hub of the cell that…

Q: What are the three key processes that Pmar-1 activates in the micromeres? Explain the importance of…

A: Micromere is a small form of the blastomere, which is formed as a result of unequal cleavage of a…

Q: The xyz gene product, encoded by a new viral strain, has coresponding human homologs. Blast analyses…

A: Scoring matrix  Scoring matrix measures the similarity between two characters in sequence alignment.…

Q: To create a cDNA library, cDNA can be inserted into vectors and cloned. In the analysis of cDNA…

A: A gene is an area of DNA molecule or polymer of amino acid that encodes function. A chromosome…

Q: What are the factor of expressed sequence tag?

A: Expressed sequence tags are small stretches of DNA sequences that are typically 100 to 800…

Q: Briefly explain why CpG islands might have come to be underrepresented in eukaryotic genomes.

A: CpG islands are the sites of methylation on DNA. Methylation of DNA occurs to silence some genes of…

Q: yeast cells, telomerase remains active and maintains telomeres of about 300 base pairs. Propose what…

A: The end areas of chromosomes are called telomeres , are repetitive sequence that protect gene from…

Q: Eukaryotic genomes are replete with repetitive sequences that make genome assembly from…

A: Tandem repeats of the dinucleotide sequence are the repetitive sequences that are found at many…

Q: You receive purified mRNA encoding the peak protein of SARS Cov-2 as well as the plasmid pET32 (a…

A: DNA cloning with plasmid vector involves the addition of a foreign gene into a plasmid vector. The…

Q: n yeast cells, telomerase remains active and maintains telomeres of about 300 base pairs. Propose…

A: Deletion is a type of mutation in the DNA (deoxyribonucleic acid) that…

Q: A 2.0kb bacterial plasmid ‘BS1030’ is digested with the restriction endonuclease Sau3A; the plasmid…

A: Plasmids are small, circular, double stranded, and extrachromosomal bacterial DNA. i.e. it is…

Q: this is what i have said about this image so far, what else can be said aswell including the raw…

A: The raw count data presented in this comprehensive gene expression analysis offers a quantitative…

Q: The following figure shows a screen shot  from the UCSC Genome Browser, focusing  on a region of the…

A: Bioinformatics is the study of biological data through computation, storage, and interpretation.…

Genomic studies

Genomic studies (genomics) is the study of the whole genome of an organism. Using bioinformatics and high-performance computing, genomic researchers examined massive amounts of deoxyribonucleic acid (DNA)-sequence data to discover variations or mutations that affect drug response, health, or disease. Genomics is a much younger discipline than genetics, having only emerged in the last few decades as a result of technological advancements in DNA sequencing and computational biology.