TAC CTA CTC TAG TTA ACCACA GTT GCCATC Transcribe the given template strand of DNA:I Second mRNA base G. UUU UCU UAU Tyr UGU Cys UUC F UCC Ser UAC M UGc (C) UAA Stop UGA Stop Phe UUA UCA (S) Leu UUG () UCG UAG Stop UGG Trp (W)G CUU CCU CAU His CGU CAC (H) CGC Arg (R) CUC Leu Ccc Pro CUA (L) CCA (P) CAA CGA Gin (Q) CUG G CAG CGG AGU AUU lle AUC (1) ACU AAU Asn Ser (N) AGC (S) ACC Thr AAC (T) AUA ACA Lys AAG (K) AGG AAA AGA Arg (R) Met M or start AUG ACG GUU GCU GAU GGU Asp GUC Val GCC Ala GAC 0) GGC Gly G. GUA (V) (A) GAA (G) GGA GCA Glu (E) GUG GCG GAG GGG Third mRNA base (3' end of codon) UCAG UCAG UCAG UCAG CA First mRNA base (5' end of codon)

A  partial DNA template has the following sequence of nucleotide bases: A-C-G-T-A-C Type the nucleotide bases for the corresponding mRNA strand.

The bacterial primase enzyme synthesizes _______________________.   a short DNA primer using RNA as a template a short protein primer using RNA as a template an RNA strand in absence of template DNA a short DNA primer using DNA as a template a short RNA primer using DNA as a template

State the characteristics that would be required for using a restriction endonuclease with human DNA, and explain why you needed to use the empty DNA fragment.

Transcribe an RNA from the following DNA Template sequence:5’-CCA ATG GCA ATG ATT-3’

Draw out the molecular structure of a double stranded DNA molecule. This molecule is 6 bp in size. Don't forget label.

Clone number in this case is number 196 as shown in the images. What is the exact length of the segment of human DNA that has been inserted into the plasmid? *report the entire length of the insert, not just the sequences matching the ends and labels of wells isn't needed for answer*

Use the plasmid to answer the following questions a) Digest the plasmid with EcoRI only and then both EcoRI and BamHi together. Fill in the table to show the fragment sizes produced.

Restriction endonucleases are bacterial enzymes that cleave duplex (double-stranded) DNA at specific nucleotide sequences. The mode of replication of the animal virus SV40 has been investigated by using restriction endonucleases that cleave SV40 DNA into a number of unique segments. Like most viruses, SV40 DNA is circular. The map positions of the 11 fragments produced by a pair of restriction endonucleases are shown on the next page. Immediately following a 5 or 10 minute pulse of radioactively labeled thymidine, labeled SV40 molecules that have completed replication during the pulse are isolated. These newly replicated DNA molecules are digested by the restriction endonucleases and the resulting fragments are analyzed for the relative amounts of pulse label they contain. The results are in the table below. Assume that at the time the label was added there was a random population of replicating SV40 DNA molecules in all possible stages of synthesis. From the information given below,…

What specifically will happen if DNA polymerase is inaccurate during DNA synthesis? Explain how this inaccuracy might affect the organism.