TABLE 10.1 Some biologically important fatty acids Common Name Systematic Name Abbreviation Structure Melting Point ("C) Saturated Fatty Acids Capric acid Decanoic acid 10:0 CH(CH,),COOH 31.6 Lauric acid Dodecanoic acid 12:0 CH3(CH2)10COOH 44.2 Myristic acid Tetradecanoic acid 14:0 CH3(CH2)12COOH CH3(CH2)1,COOH 53.9 Palmitic acid Hexadecanoic acid 16:0 63.1 Stearic acid Octadecanoic acid 18:0 CH(CH-)16COOH 69.6 Arachidic acid Eicosanoic acid 20:0 CHĄ(CH,)1,COOH 76.5 Behenic acid Docosanoic acid 22:0 CH3(CH)2COOH 81.5 Lignoceric acid Tetracosanoic acid 24:0 CH3(CH2)22COOH 86.0 Cerotic acid Hexacosanoic acid 26:0 CH,(CH,)2,COOH 88.5 Unsaturated Fatty Acids Palmitoleic acid cis-9-Hexadecenoic acid 16:1CA9 CH3(CH2)sCH=CH(CH2),COOH Oleic acid cis-9-Octadecenoic acid 18:1CA9 CH3(CH2),CH=CH(CH2),COOH 16 Linoleic acid cis,cis-9,12-Octadecenoic acid 18:2CA9,12 CH3(CH2),CH= CHCH,CH=CH(CH,),COOH Linolenic acid all-cis-9,12,15-Octadecenoic acid 18:3CA9,12,15 CH,CH,CH=CHCH,CH= CHCH,CH=CH(CH2);COOH -11 Arachidonic acid all-cis-5,8,11,14-Eicosatetraenoic 20:4cA5,8,11,14 CH3(CH,),CH= -50 acid CHCH,CH=CHCH;CH= CHCH,CH=CH(CH),COOH

The amino acid arginine ionizes according to the following scheme: (a) Calculate the isoelectric point of arginine. You can neglect contributions from form I. Why? (b) Calculate the average charge on arginine when pH = 9.20. (c) Is the value of average charge you calculated in part b reasonable, given the pI you calculated in part a? Explain your answer

(b) The structure of a drug called tirbanibulin which is used to treat a pre-cancerous skin condition called actinic keratosis is shown below. (i) (ii) (iii) (iv) ہے مههممنن State Lipinski's Rule of Five (ROF) and Veber's addition to the ROF. Briefly state why the ROF is used in medicinal chemistry?. Given that the molecular mass of tirbanibulin is 431.536 g.mol-¹ and its log P is 3.2, apply the ROF and Veber's additional rule to this drug and show clearly how you arrived at your answer. Comment on the outcome of your analysis of the structure in part (iii).

After staining an SDS-PAGE gel with Coomassie Blue G-250, the protein bands are visualized by de-staining the gel in a Coomassie Blue G-250 de-staining solution. This solution is made up of 10% acetic acid, 50% methanol, and 40% distilled water. How much of each of these components do you need to prepare 5 liters of Coomassie Blue G-250 de-staining solution?

Determine the sequence of the following 10-mer of ferritin using the data below. i. Complete acid hydrolysis of the 10-mer results in identification of 1A, 1D, 1E, 1F, 1H, 1K, 2L, 1T, 1Y ii. Digestion of the 10-mer with chymotrypsin results in 3 fragments containing (D, H, K, T), (F, L) and (A, E, L, Y) iii. Digestion of the 10-mer with V8 protease, which cuts on the C-side of acidic amino acids, results in three fragments containing (D, F, L, Y), (A, E, L) and (H, K, T) iv. Digestion with trypsin results in a fragment H-T and a fragment with all the other amino acids v. Treatment of the 10-mer with FDNB followed by complete acid hydrolysis results in the following two derivatives, DNP-1 and DNP-2 vi. Treatment of the 10-mer with a carboxypeptidase results in a free threonine