Use the pka values, calculate the isoelectric point (pl) of the following amino acids.a) Leucineb) Argininec) Aspartic acidd) Glutamic acidAmino AcidAlanineArginineAsparagineAspartic acidCysteineGlutamic acidGlutamineGlycineHistidineIsoleucineLeucineLysineMethioninePhenylalanineProlineSerineThreonineTryptophanTyrosineValinepk, ofa-COOH2.352.012.022.102.052.102.172.351.772.322.332.182.282.582.002.212.092.382.202.29pk ofa-NH,*9.879.048.809.8210.259.479.139.789.189.769.748.959.219.2410.609.159.109.399.119.72pk ofSide Chain12.48-3.868.004.076.10-10.53II10.07-

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Answered: Use the pka values, calculate the…

Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

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Ceruloplasmin is a blue-colored monomeric oxidase found in mamma- lian blood plasma. It contains multiple copper atoms per mole- cule. A ribbon representation of its three-dimensional shape is shown below: a) b) c) Identify any cofactor(s) which ceruloplasmin may possess. How do you know? Does ceruloplasmin act as an enzyme ? Describe the secondary, tertiary and quaternary structures of ceruloplasmin.
3) The following is the amino acid sequence of bradykinin, a peptide released by certain organisms in response to wasp stings: Arg – Pro – Pro - Gly – Phe – Ser – Pro – Phe – Arg What amino acids or peptides are produced when bradykinin is treated with each of the following reagents? a) with Carboxypeptidase c) with Trypsin b) with Chymotrypsin d) with DNFB
Amino acid analysis of the peptide angiotensin II shows the presence of eight different aminoacids in equimolar amounts: Arg, Asp, His, Ile, Phe, Pro, Tyr and Val. Partial hydrolysis ofangiostensin II with dilute HCl yields the following fragmentsa.) Asp –Arg- Val –Tyr b.) Ile –his –Proc.) Pro –Phe d.) Val – tyr – Ile –His a.)What is the sequence of angiostensin II and what Fragment would result if angiotensin II were cleaved with trypsin? Withchymotrypsin?
A decapeptide was isolated from a thermophilic bacteria. Treatment of this peptide with 2- mercaptoethanol gave 2 pentapeptides. Further analysis of this peptide gave the following data: Trypsin: (M,Y), (K,Y,M), (K, 2A, 2C) Chymotrypsin: (2K, 2A, 2C, Y), (M), (Y,M) What is the primary structure of the decapeptide? Thank you.
Draw the L(leucine)-A(alanine)-E(glutamate) triple tide and calculate its isoelectric point.
Which peptide has greater absorbance at 280 nm? Why? a) Gly-Ala-Trp-Lys-Glu-Tyr-Hisb) Ser-Val-Phe-Gly-Ala-Leu-Ilec) Gln-Leu-Glu-Phe-Thr-Leu-Tyr
Creatine phosphate is used as a phosphoryl donor for ATP synthesis in muscle. It provides ATP for the initial few seconds of intense exercise. a) What enzyme catalyzes the synthesis of creatine phosphate? Write out the reaction. b) For a skeletal muscle at rest, the following metabolites are present at the indicated concentrations. [ATP] = 4 mM; [ADP] = 0.013 mM [creatine phosphate] = 25 mM; [creatine] = 13 mM Calculate the AG for the creatine kinase reaction for a muscle at rest.
Draw the Fischer projections representing the L forms of the following amino acids at pH higher than their isoelectric point. a) cysteine b) alanine
1) Draw a chromatogram depicting separation of proteins 1, 2, and 3 (protein #1, mw 30,000, protein #2, mw 60,000, and protein #3, mw 90,000). b)Indicate on the chromatogram in (a) where insulin would elute. Would insulin have total accessibility, some accessibility or no accessibility to the particle pores on this column? c)Referring to the size exclusion chromatogram in (a), indicate on the chromatogram where you would expect a molecule with a size of 40,000 to elute. Would you expect the molecule to be well resolved, somewhat resolved, or not resolved from the peak for protein #1?
Mark the CORRECT alternative regarding the amino acid tyrosine: a) It can present anionic forms with two different charge magnitudes at pH values above the isoelectric point b) it is considered as a basic amino acid c) pH 7 migrates towards the cathode d) the side chain is not part of the acid-base equilibria that take place in aqueous solution e) exhibits minimal solubility in water at a pH value greater than 7
The dissociation of lactic acid to lactate is shown in the reaction. Lactic acid has a pKa of 3.86. OH Lactic acid OH OH Lactate + H+ A solution containing a mixture of lactic acid and lactate was found to have a pH of 5.10. Calculate the ratio of the lactic acid concentration to the lactate concentration in this solution. [lactic acid]/[lactate] =
An oligopeptide has the following amino acid sequence: NH2-Ala-Glu–Leu–Trp–Tyr-Ser–Gly–Lys–Leu-Ala–Arg-Ala-Phe-Ile-Pro–Gly-COOH a) Estimate the net electric charge of the molecule at pH 8.0 and pH 11.0. b) If the above peptide is passed through a cation exchange chromatographic column (that is, the matrix of the column has negative charges) stabilized at pH 8.0, would you expect it to be retained on the column? c) Indicates the number of fragments, and the sequence of each of them, that would be obtained when treating the peptide in question with: i) trypsin, ii) chymotrypsin.

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Use the pka values, calculate the isoelectric point (pl) of the following amino acids. a) Leucine b) Arginine c) Aspartic acid d) Glutamic acid