Sort the following steps of GPCR signal propagation (you may not use all the steps): A) Dephosphorylation of GPCR B) G protein dissociation to adenylate cyclase and GTP binds to the G protein C) Ligand binding to GPCR D) G protein dissociation from GPCR and GDP binds to the G protein E) Synthesis of CAMP F) Activation of PKA G) G protein association to adenylate cyclase H) G protein dissociation from GPCR and GTP binds to the G protein O F, C, H, F, B OB, D, A, E, H O C, H, G, E, F A, G, H, B, E

1) Amino acids that contain hydroxyl groups in their R-groups can be phosphorylated during cellular signaling. (a) What amino acids can be phosphorylated? (use the one letter amino acid abbreviations) (b) Does phosphorylation turn a signaling protein “on” or “off”? (c) Whatis the most common source of a phosphate used to phosphorylate a protein (d) What enzyme type (or category of enzymes)removes phosphates from proteins?

Yeast cells are eukaryotes, and they require a functional secretory pathway to grow and to maintain cellular organization. But surprisingly, when SRP is removed from yeast cells by deleting the relevant genes, the cells are still alive (although they grow slowly). a) How can yeast survive without SRP? Propose two alternative hypotheses. b) How might electron microscopy of normal and SRP-deficient yeast cells help you to distinguish between these two hypotheses?

If you wanted to engineer the initiator caspase from the Fas/FasL extrinsic apoptosis pathway to function in the intrinsic pathway (e.g. in response to DNA damage), which of the following would be the most likely approach to succeed? O Replace the extrinsic pathway's initiator caspase Death Effector Domain with a CARD domain O Replace the Fas Death Domain with a cytochrome C binding domain O Generate a version of the Fas receptor that localizes to the mitochondrial intermembrane space 80 O Replace the intrinsic pathway's initiator caspase CARD domain with a Death Effector Domain F3 0 $ 4 DOD DOO R F4 S % 5 T No new data to save. Last checked at 3:01pm Submit Qu B4 ♫ 8 tv A F5 < 6 MacBook Air F6 Y & 7 F7 * 8 X F8 ( 9