Shikha needs to complete a serial dilution of an immortal cell line that she needs to make sure does not overgrow. Unfortunately, her lab is down to the last bottle of sterile media, and inside it there is only 40 mL left. Her SOP tells her she needs to do five 1:10 serial dilutions, with each serial dilution made up of total 10 mL. She does as many serial dilutions as she can while still making each serial dilution up to total 10 mL. If her original cell concentration is 50 million cells, what is the final concentration after doing the last serial dilution?

Shikha needs to complete a serial dilution of an immortal cell line that she needs to make sure does not overgrow. Unfortunately, her lab is down to the last bottle of sterile media, and inside it there is only 40 mL left. Her SOP tells her she needs to do five 1:10 serial dilutions, with each serial dilution made up of total 10 mL. She does as many serial dilutions as she can while still making each serial dilution up to total 10 mL. If her original cell concentration is 50 million cells, what is the final concentration after doing the last serial dilution?

Basic Clinical Laboratory Techniques 6E

6th Edition

ISBN:9781133893943

Author:ESTRIDGE

Publisher:ESTRIDGE

Chapter2: Basic Hematology

Section2.12: Reticulocyte Count

Problem 5RQ

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