There are 10 E. coli cells in a test tube.  After 4 generations in exponential phase, how many E. coli are there?

Q: You are developing a hypothetical culture medium for the isolation of salmonella Typhimurium. How…

A: Salmonella typhimurium is a gram-negative bacteria and are found in the lumen of intestine. They are…

Q: Why is the plate count method a determination of the number of viable cells?

A: The plate count method, also referred to as the viable count method or colony-forming unit (CFU)…

Q: Use the following data to calculate the original bacterial sample. 1 ml Sample of E. coli 1/10 OF-10…

A: Bacteria is a ubiquitous microscopic prokaryotic organisms which does not contain true nucleus and…

Q: How do we calculate the generation time of a bacterial culture that increases in population of 104…

A: Generation time is defined as the time between two consecutive generations of a population.  With…

Q: 6a. A sample is given to you that contains 6 million cells per ml.  You must make a series of serial…

A: A log dilution is a tenfold dilution, meaning the concentration is decreased by a multiple of ten.

Q: Using phages that came from E. coli with the genotype al+ be+, you infect a strain ofE. coli that is…

A: Auxotrophs are the term used for the organism or bacteria that cannot express a gene due to mutation…

Q: How could you isolate mitochondria from liver cells using differential centrifugation?

A: Isolated mitochondria are used to perform several functions. This organelle is used to study…

Q: In a plaque assay, a phage has undergone _________ cycle when plaques are observed on the agar lawn…

A: Viruses are obligate intracellular parasites that requires a living host cell to grow and multiply.…

Q: Determine the appropriate bacterial strain that can be allowed to mate with the strain that is…

A: An organism's genotype is influenced by its genome as well as by its environment. It is not as…

Q: Is the isolation plate pigmented? (is their a color for it)? what is colony morphology(single cells…

A: Colony morphology is a method that is used to describe the characteristics of an individual colony…

Q: 1) What are all of the different colony types that can be seen on a mixed-culture streak plate?  2)…

A: Hi! Thanks for your question. As you have posted multiple questions, I am answering only the first…

Q: how do you graph change over time for onion, cheek , and bacterial cell? Can you explain how you…

A: Onion cells, human cheek cells, and bacterial cells are subjected to be affected by any change in…

Q: You decide to knock-out URA3 and replace it with NeoR. What type of media will you plate your yeast…

A: * knockout with URA3 and replace it with NeoR. * URA3 URA3 is a gene on chromosome V present in…

Q: Why is it necessary to sterilize the loop between streaks when streaking for single colonies?

A: Streaking is a technique used to isolate a pure strain from a single species of microorganism, often…

Q: Can you be sure that after 6.5 hours all the cells are viable? What would you do to determine the…

A: Cell viability refers to the proportion of live, healthy cells within a sample.The measurement of…

Q: What are denaturing conditions?

A: There are 4 types of biomolecules- carbohydrates, fats, nucleic acids and proteins. Proteins are the…

Q: Horizontal gene transfer between bacteria occursthrough three mechanisms: transformation,…

A: The process of transmission genes from one organism to another is termed as gene transfer. There are…

Q: Determine the appropriate bacterial strain that can be allowed to mate with the strain that is…

A: An organism's genotype is influenced by its genome as well as by its environment. It is not as…

Q: LB (Lysogeny broth) medium for the growth of E. coli,

A: Pseudomonas putida is a kind of rod-shaped, Gram-negative, non-fermenting bacterium which is present…

Q: Complete the following statement about conjugation. Bacterial confirmation is the equivalent of…

A: Bacterial conjugation is the process of transfer of genetic material from one bacterium to another…

Q: Why would you want to limit the number of times you passage a cell culture line?

A: Cell culture is the process through which cells are cultivated under controlled conditions,…

Q: Describe the appearance of an S. agalactiae colony grown on blood agar. Describe how that colony…

A: The initial identification of the enterococcal and streptococcal species is based on their hemolytic…

Q: What would be the evolutionary advantage of having a period of natural “competence” in a bacterial…

A: The transfer of foreign DNA from the surroundings into a bacterial cell and those bacterial cells…

Q: 10) With the disk test, what specifically are you looking for to know if a bacteria is susceptible…

A: The disk test is done to find which bacteria is susceptible to which antibiotic. This test is also…

Q: A food sample was cooked. The bacterial population in the food decreased from 500,000 cells to 5…

A: A Decimal Reduction Time (D-value) is a term that describes how long it takes to lower a number by…

Q: In a rapidly changing environment, which bacterial population would likely be more successful: one…

A: In a rapidly changing environment, the availability of nutrients, climatic conditions keep on…

Q: Does the addition of E. coli change the dilution in the calculation of PFU/mL in the original phage…

A: Plaque refers to the clear or visible zone formed by the viral sample when it is introduced to the…

Q: What is the principle behind invertase test

A: Invertase was an enzyme that hydrolyses the disaccharide sucrose into monosaccharides glucose and…

Q: A protocol calls for 10^3 phage particles as starting material. How much of your phage suspension…

A: Bacteriophage -- Phage means to eat .Bacteriophages are viruses which infect bacteria and phage…

Q: Based on the evidence provided, which of the following graphs is most appropriate to use to show the…

A: As per the given data,  Yeast cells were grown on 5% glucose media on a sterile petri dish for 5…

Q: If you counted 300 colonies on a plate that had a 10-5 dilution of bacteria culture, how many…

A: Introduction  Colonies are groups of microorganisms, such as bacteria or yeast, growing on or within…

Q: is staphylococcus aureus cells under 40 times magnification bigger than onion cell or cheek cell?

A: Staphylococcus aureus is a Gram-positive, round-shaped bacterium that belongs to the Firmicutes and…

Q: T4 phage isolated from E. coli strain B infect E. coli strain K,

A: T4 is a capable of undergoing only a lytic cycle  the phage initiates an E.coli infection by…

Q: Imagine you repeated the counting of N. crassa conidial cells and found an average number of cells…

A:

Q: What are the specific E. coli strains used to make competent cells? Explain the characteristics of…

A: Competent cells are the ones that are prepared to easily take-up modified plasmids (containing…

Q: For the following sets of partial diploid bacteria, how do I fill out this table? I am not sure how…

A: The method of identifying bacteria frequently includes observing their behavior beneath diverse…

Q: Determine the appropriate bacterial strain that can be allowed to mate with the strain that is…

A: An organism's genotype is influenced by its genome as well as by its environment. It is not as…

Nutrient Growth Medium

It is a culture medium or nutrient broth for all kinds of microorganisms to grow properly and create their colony. This matrix provides support to increase the population of microbes through the method of cell proliferation. It is regarded as the food material for those organisms. It is a type of in vitro mechanism, in which the development of life is processed outside the host body. It can be solid, semi-solid, or liquid in nature. Different kinds of cells develop in different types of media.