Samples came to the laboratory for analysis. You are asked to identify the differences between these examples. It is known that the DNA of the samples must first be isolated in order to understand the difference. Isolated to DNA. It's time for the next step. It was decided to use the RAPD technique. The amount of DNA in the samples isolated in the spectrophotometer was determined. The sample is measured at 250 ng / microliter. In the RAPD technique, the total reaction volume for PCR is optimized to be 15 microliters. This is adjusted to contain 5 microliters of DNA in 15 microliters. This technique requires 25 ng of DNA to work. How to dilute the measured 250 ng / microliter sample DNA into the appropriate amount for the reaction to occur?

Human Heredity: Principles and Issues (MindTap Course List)
11th Edition
ISBN:9781305251052
Author:Michael Cummings
Publisher:Michael Cummings
Chapter14: Biotechnology And Society
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Samples came to the laboratory for analysis. You are asked to identify the differences between these examples. It is known that the DNA of the samples must first be isolated in order to understand the difference. Isolated to DNA. It's time for the next step. It was decided to use the RAPD technique. The amount of DNA in the samples isolated in the spectrophotometer was determined. The sample is measured at 250 ng / microliter. In the RAPD technique, the total reaction volume for PCR is optimized to be 15 microliters. This is adjusted to contain 5 microliters of DNA in 15 microliters. This technique requires 25 ng of DNA to work. How to dilute the measured 250 ng / microliter sample DNA into the appropriate amount for the reaction to occur?

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