s) In two experiments carried out in a research lab, an enzyme reaction is run with and without an inhibitor. Samples were taken over time to measure the concentration of product as the reaction proceeds, with raw data given below. Data were collected at 34 °C and pH 7.5, using [S] = 0.115 M (for both trials) & [E]. = 0.0024 M (for both trials): Product concentration (mol P/L) No inhibitor [I] = 0.019 M 0 0 Time min 0 3.5 9 13 17 26 43 69 104 156 208 311 415 519 0.0016 0.0038 0.0052 0.0078 0.0111 0.0180 0.0281 0.0412 0.0525 0.0587 0.0598 0.0600 0.0600 0.0011 0.0023 0.0031 0.0045 0.0074 0.0119 0.0195 0.0271 0.0410 0.0479 0.0550 0.0588 0.0600 A. Determine the initial reaction velocities for both reactions. 3. From these data, can you determine what type of inhibition is occurring? If so, show evidence of the type of inhibition occurring. If not, describe what experiment(s) should be done to determine the type of inhibition (competitive, non-competitive or uncompetitive), as well as how the data should be treated to find the inhibitor type.
s) In two experiments carried out in a research lab, an enzyme reaction is run with and without an inhibitor. Samples were taken over time to measure the concentration of product as the reaction proceeds, with raw data given below. Data were collected at 34 °C and pH 7.5, using [S] = 0.115 M (for both trials) & [E]. = 0.0024 M (for both trials): Product concentration (mol P/L) No inhibitor [I] = 0.019 M 0 0 Time min 0 3.5 9 13 17 26 43 69 104 156 208 311 415 519 0.0016 0.0038 0.0052 0.0078 0.0111 0.0180 0.0281 0.0412 0.0525 0.0587 0.0598 0.0600 0.0600 0.0011 0.0023 0.0031 0.0045 0.0074 0.0119 0.0195 0.0271 0.0410 0.0479 0.0550 0.0588 0.0600 A. Determine the initial reaction velocities for both reactions. 3. From these data, can you determine what type of inhibition is occurring? If so, show evidence of the type of inhibition occurring. If not, describe what experiment(s) should be done to determine the type of inhibition (competitive, non-competitive or uncompetitive), as well as how the data should be treated to find the inhibitor type.
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
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Question
![s) In two experiments carried out in a research lab, an enzyme reaction is run with and without an inhibitor.
Samples were taken over time to measure the concentration of product as the reaction proceeds, with raw data
given below.
Data were collected at 34 °C and pH 7.5, using [S] = 0.115 M (for both trials) & [E], = 0.0024 M (for both trials):
Product concentration (mol P/L)
No inhibitor [1] = 0.019 M
0
0
0.0011
0.0023
0.0031
0.0045
0.0074
0.0119
0.0195
0.0271
0.0410
0.0479
0.0550
0.0588
0.0600
Time
min
0
3.5
9
13
17
26
43
69
104
156
208
311
415
519
0.0016
0.0038
0.0052
0.0078
0.0111
0.0180
0.0281
0.0412
0.0525
0.0587
0.0598
0.0600
0.0600
A. Determine the initial reaction velocities for both reactions.
B. From these data, can you determine what type of inhibition is occurring?
If so, show evidence of the type of inhibition occurring.
If not, describe what experiment(s) should be done to determine the type of inhibition (competitive,
non-competitive or uncompetitive), as well as how the data should be treated to find the inhibitor type.](/v2/_next/image?url=https%3A%2F%2Fcontent.bartleby.com%2Fqna-images%2Fquestion%2F6fb3429f-a406-4812-82fc-346d6a20f662%2F2f7cb8b3-1cbb-45f4-a9d3-ec54995f88b5%2Fj3ibnt_processed.png&w=3840&q=75)
Transcribed Image Text:s) In two experiments carried out in a research lab, an enzyme reaction is run with and without an inhibitor.
Samples were taken over time to measure the concentration of product as the reaction proceeds, with raw data
given below.
Data were collected at 34 °C and pH 7.5, using [S] = 0.115 M (for both trials) & [E], = 0.0024 M (for both trials):
Product concentration (mol P/L)
No inhibitor [1] = 0.019 M
0
0
0.0011
0.0023
0.0031
0.0045
0.0074
0.0119
0.0195
0.0271
0.0410
0.0479
0.0550
0.0588
0.0600
Time
min
0
3.5
9
13
17
26
43
69
104
156
208
311
415
519
0.0016
0.0038
0.0052
0.0078
0.0111
0.0180
0.0281
0.0412
0.0525
0.0587
0.0598
0.0600
0.0600
A. Determine the initial reaction velocities for both reactions.
B. From these data, can you determine what type of inhibition is occurring?
If so, show evidence of the type of inhibition occurring.
If not, describe what experiment(s) should be done to determine the type of inhibition (competitive,
non-competitive or uncompetitive), as well as how the data should be treated to find the inhibitor type.
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