A purified protein fraction has a total sample volume of 360 µL. The sample has a corrected A280 of 0.484, and the blank corrected A280 was 0.052. (Both values were measured with a path length of 1.00 cm.) If 5.00 µL of the sample was used in a reaction, calculate the mass of protein in the reaction (in µg).

The mass of protein can be identified by calculating the concentration of sample first. Using the following formula to estimate protein concentration, where path length is 1 cm. Concentration (mg/ml) =A280 (Absorbance at 280nm)/ path length (cm) Given that A280 of protein sample = 0.484 Concentration= 0.484 / 1 Concentration = 0.484 mg/ml This means that: 1ml of sample has 0.484 mg of protein 1ml= 1000 µL and 1mg = 1000 µg so, 1000 µL has 0.484 x 1000 ug of protein 5µL will have 0.484 x 10001000x 5= 0.484 x 5= 2.42 µg The mass of protein is 2.42 µg.

Answered: A purified protein fraction has a total…

Human Anatomy & Physiology (11th Edition)

11th Edition

ISBN:9780134580999

Author:Elaine N. Marieb, Katja N. Hoehn

Publisher:Elaine N. Marieb, Katja N. Hoehn

Chapter1: The Human Body: An Orientation

Section: Chapter Questions

Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...

See similar textbooks

Related questions

Concept explainers

Question

100%

A purified protein fraction has a total sample volume of 360 µL. The sample has a corrected A₂₈₀ of 0.484, and the blank corrected A₂₈₀ was 0.052. (Both values were measured with a path length of 1.00 cm.) If 5.00 µL of the sample was used in a reaction, calculate the mass of protein in the reaction (in µg).

Expert Solution

Trending now

This is a popular solution!

Step by step

Solved in 2 steps

SEE SOLUTION Check out a sample Q&A here

Knowledge Booster

Learn more about

Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, biology and related others by exploring similar questions and additional content below.

Similar questions