What is the purpose of adding mineral oil to an O/F Media tube and why do we only put mineral oil in only one?

Q: Which of the following is the basic difference between serological (blow-out) and measuring…

A: A pipette is a sort of laboratory instrument that is frequently used in biology and chemistry to…

Q: What is the purpose of sealing the flask with a balloon? What product is captured in the balloon?

A: Fermentation is the process of ATP generation by the microorganism. Carbohydrates are the substrate…

Q: How would you prepare a 1:500 dilution? You cannot use more than 10mL per tube.

A: In scientific research and experimentation, it is often necessary to dilute substances to achieve…

Q: what does chroomatography observation mean

A: Column fractionation was developed in the 1890s as a technique of separating the various components…

Q: why is the tube passed through the flame after removing and before replacing the cap

A: It is a aseptic technique follows during the inoculation of pure culture of microorganism. This…

Q: In using an ultracentrifuge, arrange the following steps in correct order A. Turn the knob to the…

A: Centrifugation is a techniques in which involves centrifugal force to separate the particles from…

Q: What is this test? What coated the bottom of the wells? What were we testing for? How did we know it…

A: The test is ELISA. It is an Enzyme linked Immunosorbant Assay, which is used in many applications.…

Q: What is the result of tube A in this Voges-proskauer test? What are the reagents of this test? What…

A: Voges-proskauer test is performed to differentiate between facultative anaerobic enteric bacteria.

Q: Given: The used ocular objective while taking this image, has magnifying power of 6x The used…

A: Given : Stage micrometer used has graduation = 0.01mm Ten graduation on ocular micrometer coincides…

Q: explain why it is not good to break sterile field  when inserting a foley catheter

A: Inserting the indwelling catheter is an invasive procedure. Sterile technique should follow while…

Q: Why Purified synthetic alcohol is preferred for level vial?

A: An alcohol is an organic compound that carries at least one hydroxyl functional group (-OH) bound to…

Q: Give two ways to prevent the diffusion of oxygen into the medium in this test.

A: The main two ways to prevent the diffusion of oxygen are given below.

Q: Why is it important to eject a small amount of blood from the syringe sample onto a gauze pad before…

A: Blood gas analysis is a diagnostic test. It gives useful information regarding the respiratory and…

Q: Why is a metal loop heat to read before picking up a bacterial sample? What should you do with a…

A: In microbiology, inoculation is the technique of transferring bacteria into culture media so that…

Q: explain why it is important to maintain sterility when inserting a foley cathether

A: Foley's catheter is an indwelling catheter, i.e. it can be left in place for longer durations than…

Q: What are the advantages and disadvantages of the filter method in the analysis of water samples?

A: Utilising the membrane filter method, test is gone through the layer utilising a filter funnel and…

Q: Jessie is working with a 100% stock concentration of Clorox. She decides to add 100 μL of bacteria…

A: For calculating the unknown concentration of the tube we will be using the formula as mentioned…

Q: What is the difference between normal and reverse phase chromatography?

A: The main Difference between Normal and reverse phase chromatography is 1) In normal phase…

Q: If each agar plate contained 20 ml of LB agar and had an ampicillin concentration of 150 ug/ml, what…

A: Given data:  Each plate contains-  LB agar=20ml Ampicillin= 150 micrograms per ml.  Amount of…

Q: what is the purpose of the FeCl3 in this aspirin test?

A: Now, setting up and observing the breakdown:1) Divided: We split apirin into two tubes, one with HCl…

Q: In step 3 of today's protocol, it mentions that the incubation step with the P2 buffer should not be…

A: P2 buffer is a lysis buffer solution containing sodium dodecyl sulfate to puncture holes in cellular…

Q: What are the equipments/ materials used in imoobilizing and separating cells when using: spread…

A: Aseptic techniques refer to any method which is used to sterilize and maintain sterility of the work…

Q: If an OD=1.0 culture yielded a plate count of 2.2 x 107 cfu/mL. What is the cell count of an OD=0.5…

A: Answer: The relationship between colony-forming units per millilitre (cfu/mL) and optical density…

Q: Explain why the following step are necessary during subculturing:  Flaming the neck of…

A: Aseptic technique-Aseptic technique should be observed whenever microorganisms are transferred from…

Q: Why is UV light used in Column chromatography experiment ?

A: A chromatography procedure used to separate a single chemical compound from a mixture is known as…

Q: chemical(s) best at inhibiting both Gram- negative and Gram-positive bacteria [ Choose ] chemical…

A: Bacteria are microscopic, unicellular, prokaryotic organisms found in diverse environments. They can…

Q: the step by step process of Nitrate Redution Test using a schematic diagram

A: Nitrate reduction test This test is widely used in microbiology laboratories to determine the…

Q: Which is more accurate a transfer pipette or a measuring pipette? How are these compared to a…

A: Features Transfer pipette Measuring pipette MicropipettePurposeUsed for simple liquid transfers…

Q: What is the principle of counting with a hemocytometer? What are the advantages and disadvantages?

A: Answer: HEMOCYTOMETER : It is the tool used in most of the biochemistry and biology labs in order to…

Q: How to use the sterile filter? Show the different sizes and specifications of sterile filters.

A: Sterilization is the method that is used to eliminate all forms of life and biological agents…

Q: Prepare the following LB media with antibiotic added. A 100ml of LB media with 25 micro/ml of Amp…

A: Luria-Bertani (LB) broth is a liquid media comprised of several carbon and nitrogen sources. It is…

Q: Explain how to conduct a Kirby-Bauer disk diffusion test. Include all of the steps involved

A: INTRODUCTION Kirby - Bauer Disc diffusion method Kirby - bauer disc diffusion method is widely known…

Q: Distance of color diameter (mm) 20 15 KMNO, 0.1M - KMNO, 1.0M - 5. 15 45 09 75 Time (minutes) 06

A: * The molecules movement in  agarose gel is dependent on the  size  charge  *And pore size also…

Q: Are TSA, MRS and MOX differential media? Why or why not?

A: In microbial biology, differential media refers to any growth media that comprises of certain…

Q: What is the purpose of inverting (or siding up) an inoculated plate when we place it inside…

A: The incubator is used for the incubation of Petri plates. Petri plates are used to grow the…

Q: When loading samples onto a gel, why should expelling all the liquid in the tip be avoided?   a)All…

A: It is a laboratory tool commonly used in biotechnology and medicine to transport a measured volume…

Q: Why must a discard tube should be collected first before an anticoagulated tube when using a…

A: Venipuncture is the most common method of blood collection. Blood is normally drawn from the medial…

Q: You are determining the concentration of your culture, and have counted the following on your…

A: Hemocytometer is a glass slide at with margins on its surface which is used for counting the number…

Q: How does concentration of the buffer affect current in electrophoresis? What are the implications on…

A: In electrophoresis if electric current is applied to ions of a medium then the ions will move within…

Q: Jessie is working with a 100% stock concentration of Clorox. She decides to add 100 µL of bacteria…

A: The scenario involves working with a 100% stock concentration of Clorox, bacteria, and a nutrient…

Q: In aseptic technique, what will happen if the inoculated solid plated media is not inverted prior to…

A: Culture Media   Culture media also known as growth media may be liquid,  semisolid, or solid, is use…

Inheritance and Mendelian Genetics

Inheritance means the passing of traits to offspring from parents. These traits could be passed either through asexual reproduction or sexual reproduction. The offspring receives the genetic material from the parents. Mendelian inheritance is a certain biological inheritance that follows the rules and principles laid down by Gregor Mendel in 1865. The initial hypothesis was considered wrong, but other inheritance theories modified the concept of inheritance and thus genetics.