CITRATE TEST This test determines the ability of bacteria to use citrate as source of carbon and inorganic ammonium salt as a source of nitrogen. The following are the stepwise procedure in conducting citrate test: 1. Infuse the Simmons citrate agar on the slant by touching the tip of sterilized needle to a colony. 2. Incubate at 37 degree Celsius for 24 to 48 hours. A positive result is indicated by the pH indicator, bromothymol blue which turns the growth green to blue.   Task: In a tabulated form, determine if the Pseudomonas aeruginosa, are positive or negative with

Human Anatomy & Physiology (11th Edition)
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ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
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CITRATE TEST
This test determines the ability of bacteria to use citrate as source of carbon and
inorganic ammonium salt as a source of nitrogen. The following are the stepwise procedure
in conducting citrate test:
1. Infuse the Simmons citrate agar on the slant by touching the tip of sterilized needle
to a colony.
2. Incubate at 37 degree Celsius for 24 to 48 hours.
A positive result is indicated by the pH indicator, bromothymol blue which turns the growth
green to blue.

 

Task:

In a tabulated form, determine if the Pseudomonas aeruginosa, are positive or negative with
citrate test.

Interpret your answer and include the reaction of organism on this test.

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