Question one: PCR You want to amplify the underlined sequer 1) Design Forward and Reverse primers 2) Calculate the melting temperature for

Q: COMPONENT 1 REACTION N+1 REACTIONS 15.80 µL 2.50 µL 1.50 µL 2.00 µL 1.00 µL 1.00 µL 0.15 µL…

A: Polymerase chain reaction is a procedure that is used to amplify a particular segment of DNA. This…

Q: QUESTION 3: Why does a PCR reaction require a primer? composed of DNA or RNA? Would you expect this…

A: The polymerase chain reaction, or PCR, is a chemical reaction used by molecular biologists to…

Q: The laboratory has lost the protocol to follow during the PCR reaction and only give you this.…

A: PCR or polymerase chain reaction is a method used for amplifying a small DNA sample. From a small…

Q: What is real-time PCR?

A: Besides tools, there a several techniques which are used in recombinant DNA technology. These…

Q: Recombinant pharmaceuticals (for the production of insulin, human growth hormone or blood clotting…

A: The human genome is composed of sequences of nucleic acids, which are encoded as DNA(Deoxyribo…

Q: Describe and contrast the common steps of DNA replication in vivo and the PCR reaction in vitro? In…

A: DNA replication and Polymerase Chain Reaction are both processes that create copies of DNA. DNA…

Q: What is experiment of Thermodynamics of Small Oligomeric Duplex DNA Denaturation.

A: Double-stranded DNA is the most common kind of DNA molecule found in nature. The strands can be…

Q: Why is it necessary to chelate the metal ions from the solution during the boiling/lysis step at 100…

A: The polymerase chain reaction (PCR) is a fundamental biochemical method for exponentially…

Q: ... and ..... are in product of a pcr reaction:   primers   nucleotides   both    none

A: Introduction :- PCR is described as the polymerase chain reaction . It is a bio-physical technique…

Q: You receive a tube containing 18.8 nmol of lyophilized (freeze dried) primers to be used in PCR. How…

A: Primers are a short nucleic acid sequence that provides a starting point for DNA synthesis. I…

Q: How do you know if it works? If you have more than one band in a lane, why what would be the reason

A: PCR is a polymerase chain reaction and it is used to amplify the segment of DNA/Nucleic acid.

Q: What are the importance of accurate wavelength measurement in purified DNA sample?

A: DNA is a double helical hereditary molecule. The nucleosome is a monomer of the DNA.  In DNA, four…

Q: What are the roles of the following reagents in DNA extraction? a. Ethanol b. NaCl c. SDS d. TE…

A: A) The initial role of the ethanol and monovalent cations is to remove the solvation shell…

Q: Question 1. Restriction endonucleases can be isolated from a number of bacteria. In bacteria…

A: Restriction endonucleases are DNA cutting enzymes. They cleave the DNA at or near specific sequence…

Q: Question 1 During nucleic acid hybridization, the probe is labelled for DNA stability to increase…

A: Hybridization permits the distinguishing and cloning of explicit qualities, investigation of levels…

Q: Tell me how did you prepared the gel if the tray is 150 ml? how many milliliters of the TBE and how…

A: The answer to the first question is, 3 grams of agarose in 150 ml of TBE.   *to get the second…

Q: Why different temperatures are used in a PCR reaction?  Please answer at your own words.

A: The full form of PCR is polymerase chain reaction. Polymerase chain reaction is a process by which…

Q: Assume the samples in this diagram of gel electrophoresis are DNA molecules. Why do the samples…

A: Gel electrophoresis is used to separate molecules of different sizes. It uses charged molecules that…

Q: Recombinant pharmaceuticals (for the production of insulin, human growth hormone or blood clotting…

A: The recombinant DNA technology (cloning) is used for the production of insulin, growth hormone (GH),…

Q: Sequencing reactions are done in separate tubes for each ddNTP with a radioactive primer. Which…

A: In our Desire primer it  is 15 nucleotide long however on gels there are only 10 bands are present…

Q: What is the first step that should be done in PCR testing?

A: PCR is the polymerase chain reaction. PCR test or polymerase chain reaction test is good method to…

Q: Our PCR samples already contain loading dye, but sometimes this isn’t the case. If your samples…

A: Gel electrophoresis is a process that helps in the separation of DNA fragments based on sizes with…

Q: Question 2. It is your first week working in the lab but unfortunately you program the PCR machine…

A: PCR is also called as polymerase chain reaction which has the ability to make millions to billions…

Q: What are the DNA extraction methods? Explain each one.

A: Ans: The Deoxyribonucleic acid (DNA) needs to be extracted out of cell for further studies on which…

Q: Please answer these two questions regarding PCR:   a) Why do you need to perform PCR on DNA obtained…

A: The polymerase chain reaction (PCR) is a technique used to make many copies of a specific segment of…

Q: Why is it important to wear gloves when setting up the PCR tubes?

A: PCR represents polymerase chain reaction which is an in vitro laboratory technique used for the…

Q: QUESTION 5 Imagine that you have cloned the gene encoding the SARS-CoV-2 spike protein to a plasmid…

A: DNA cloning is the process of making multiple, identical copies of a particular piece of DNA. In DNA…

Q: When using a micro centrifuge, what should you check before starting the machine? A. That the…

A: Microcentrifuge is a type of laboratory centrifuge, which is used to separate particles in…

Q: QUESTION 9 Imagine that a researcher has a cell from a genetically engineered organism and a clone…

A: PCR or polymerase chain reaction is a widely used method for amplifying a particular DNA…

Q: During agarose gel electrophoresis, why does DNA move through the gel when electric current is…

A: Gel electrophoresis is basically a method that aid in separating DNA fragments and other molecules…

Q: The concentration of your RNA solution is 1500 ng/μl. How much RNA solution do you need to use when…

A: The concentration of RNA in solution can be determined by measuring absorbance at 360 nm.

Q: Question 1 A T What is the sequence of the sample DNA submitted for sequencing given the gel…

A: The unknown DNA samples can be sequenced by the Sanger sequencing method. This method is based on…

Q: The following image is of an agarose gel. If DNA samples were loaded to this gel and the…

A: Electrophoresis is a method that uses an electric field to separate macromolecules in a fluid or gel…

Q: Why does the temperature constantly fluctuate inside the PCR machine? What is the purpose of the…

A: PCR, or Polymerase Chain Reaction, is a biological technique invented by Kary Mullis.

Q: Question 5 Match the following components with the biotechnology technique they play a role in.…

A: Recombinant DNA technology is a technique by which foreign DNA is introduced into the host. DNA…

Q: how do you prepare 200 ml of 1.5 agarose gel in PCR?

A: Agarose is an uncharged, inert substance that does not bind to or react with molecules. Agarose is…

Q: What factors could explain a transformation efficiency?

A: Transformation efficiency is the efficiency with which a bacterial cell can take up the…

Q: What types of DNA can be extracted? Explain your answer.

A: Some of the most common DNA extraction methods include organic extraction, Chelelx Extraction and…

Q: QUESTION 4: Should PCR primers be complementary to each other?

A: Answer 4-:PCR primers should be complementary to the strands of DNA rather than complementary to…

Q: . Draw the melting curve for primer annealing, labeling the axes. Label the position of the…

A:

Q: What is the significance of using thermostable DNA polymerase for Polymerase Chain Reaction?

A: Polymerase chain reaction (PCR) is a technique for producing multiple copies of DNA in in-vitro…

Q: You set aside some of your purified PCR product to run on a gel. Name two things we learn by running…

A: Gel electrophoresis is a process that helps in the separation of DNA fragments with the help of an…

Q: what are the reagents and other equipment needed  in polymerase chain reaction (or PCR)

A: PCR is a polymerase chain reaction which is used to amplify a given segment of DNA. PCR is a widely…

Q: Why are DNA samples that are to be separated by gel electrophoresis always loaded at the cathode end…

A: The method of electrophoresis is used to separate biomolecules based on particle charge, particle…

Q: What advantages does fluorescent labeling offer over radioactive methods of labeling DNA?

A: Fluorescent labeling and radioactive labeling have found wide applications in DNA sequence analysis…

Q: What is PCR? Why does Taq polymerase work better than a typical DNA Polymerase isolated from E. coli…

A: A groundbreaking move for molecular biology has been demonstrated by the discovery of polymerase…