The laboratory has lost the protocol to follow during the PCR reaction and only give you this. Please fill in the blanks: 94°C _______________ step for _______ minutes 52°C _______________ step for _______ seconds 72°C________________ step for ______ minutes 72°C _______________ step for ______ minutes
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A:
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- Hershey and Chase were able to see whether the backteriophage moved genetic material because they used _____________ labels to attach to sulfur and phosphorus.The subcostal short axis views are achieved by rotating the probe approximately ______ degrees anticlockwise from the subcostal 4-chamber view so that the scan plane is ______________ to the 4-chamber view and the POM is directed to the ___ o’clock position.___________ is the force that drives the migration of DNA from the _______________________________ end to the ___________ end of the gel.
- Copy and paste the link below and watch the video on Youtube https://www.youtube.com/watch?v=8RBs0Ghg_48 Answer the following Questions: 1. What are the chemicals and materials used in gel electrophoresis? 2. Draw a schematic diagram of a gel electrophoresis set-up 3. Describe the procedure in doing a gel electrophoresis experiment. Why is there a need for a leveling bubble/leveler? What is the use of the rubber dam? 4. What is the use of ethidium bromide and why must you wear gloves when you handle it? 5. What makes the DNA fragment move towards the positive plate? 6. What is the purpose of glycerol in the sample buffer? 7. What is the use of a DNA ladder? 8. What will happen when you increase the voltage of the set-up? 9. Can gel electrophoresis be used to separate amino acids? If so, how is it done?Can you please check my answer and make sure it is correct. Question: How can DNA evidence be used to convict or exonerate a defendant? Why is DNA evidence so powerful? Answer: DNA evidence can be used to perform DNA profiling to determine the genotype of the specific DNA sample. With just a small amount of DNA, PCR can produce billions of copies of that specific segment. The segments that are used are from non-coding regions that contain STR’s or short tandem repeats. These very short DNA sequences are repeated and are specific to individuals because we inherit them from our mother and father. Gel electrophoresis separates the PCR products based on their size and each band is compared to the allele ladder. This process helps to identify the alleles present in the original samples. DNA profiling is performed at many loci to be able to tell the genetic difference between different individuals with a lot of certainty. The DNA from the different suspects is compared to the allele…Copy and paste the link below and watch the video on Youtube and Answer the Questionshttps://www.youtube.com/watch?v=g-dNJdOvBM4 Polymerase Chain Reaction Questions: 1. What are the materials used for the polymerase chain reaction? 2. Draw a schematic diagram of the procedure in PCR. 3. Why is it important to design the primers at the start of the laboratory procedure? 4. What are the components of the PCR buffer and what is its pH range? What is the purpose of the buffer? 5. What is the use for magnesium chloride? 6. How much template DNA is added? What is the concentration of the primers? 7. At what temperatures does denaturation, annealing and extension occur? Why are the processes placed in that temperature? 8. In this particular PCR experiment, how many cycles was used? 9. Can this PCR be used on its own to find out if a person has Covid or not on its own? Why or why not?
- _____________ a proteomic technique that uses genetically modified yeast cells to detect protein-protein and protein-DNA interactions.The FISH technique allows researchers to localize specificDNA sequences with respect to chromosomal bands or tovisualize entire chromosomes to facilitate_____________interpretationJoe is heterozygous for a dominant genetic disease. His wife, Jenny does not have the disease. They have 5 children of which 2 (Jim and Jan) have the disease and 3 (Jose, Jerry and Julie) do not. DNA from each individual is isolated and analyzed by PCR an gel electrophoresis. 3 variable markers are studied. The results for each marker are shown here. The numbers to the left of each gel represent size in hundreds of bases Jim Jose Marker 1 Marker 2 Marker 1 Jenny Joe Marker 3 Jan Jerry Julie Jim Jose Marker 2 Jenny Joe Jan Jerry Julie Jim Jose Marker 3 Jenny Joe Based on these results, which marker is most likely associated with the condition? Jan Jerry Julie
- You are a forensic scientist in charge of DNA samples in a case where a man was murdered. You conduct gel electrophoresis on the samples to determine the DNA profile of the samples. The rows of bands have been marked to make it easier to reference the bands. The sample marked 'evidence' came from under the victim's fingernails, likely indicating a struggle. There are two suspects in the case, both of whom are brothers, they are marked suspect 1 and suspect 2, for brother 1 and brother 2, respectfully. Does the evidence collected match the DNA profiles of either brother? If yes, which one? If no, then would it be likely that the actual criminal is a family member of the brothers? If yes, what could the relation be (mom, dad, cousin, aunt, or uncle)? Note: there is a sister in their family, their parents are both still alive, and they have a robust extended family. The brothers are not twins.The gel serves as a ______________ to separate out the DNA fragmentsFind a recently developed, automated DNA extraction technique/equipment andexplain it in detail (the reference you provide must not be older than 2017).