QUESTION 34 Which of the following events does NOT occur during ERAD (ER-associated degradation)? OA. Chaperone-mediated attempts at protein folding OB. Degradation of misfolded proteins by the proteasome OC. Targeting of mis-folded proteins to the nucleus OD. Repeated attempts at protein folding QUESTION 35 Which of the following statements regarding COPII vesicles trafficking between the ER and the Golgi is NOT correct? OA. Uncoated vesicles fuse with the cis-Golgi. OB. The vesicle enclosed in a COPII coat fuses with the cis-Golgi. OC. The COPII vesicles lose the COPII coat before reaching the Golgi. OD. COPII-coated vesicles bud off from the RER. QUESTION 36 Which phenotype would you expect to see in cells that contain a defective Rab GTPase that cannot be activated on the inner surface plasma membrane? OA. The cell would lack secretory vesicles. OB. No phenotype OC. The cell would have an excess of secretory vesicles within the cytoplasm and defective secretion. OD. The cell would have an excess of secretory vesicles bound to cytoplasmic face of the trans Golgi network.

Question 28 True/False Question (suggested time - up to 1 minute): Small nuclear RNAS are essential components of spliceosomes. O True O False A Moving to another question will save tais response.

QUESTION 34 Adenylyl cyclase activity will increase in response to the binding of adenosine triphosphate protein kinase A phosphorylated CAMP activated Gs protein

Question 19 Lysogenic cells have phages that are: A actively synthesizing viral proteins B biosynthesizing only in the cytoplasm in the eclipse period D inactive in the prophage phase

Question 9 Why are integral membrane proteins difficult to study? O They are difficult to isolate in soluble form due to their hydrophobic transmembrane domains. They are difficult to isolate in soluble form due to their hydrophilic transmembrane domains. They are so small. They are so large.

QUESTION 17 A student wanted to run a protein sample on a SDS PAGE gel, and in the process of casting a gel the student had forgotten to add the stacking layer on top of the resolving layer of the gel. What problems would likely arise as a result of this mishap? Proteins would migrate much faster through the gel Proteins would not be able to migrate through the resolving layer and stays at the top of the gel Proteins will move into the resolving layer at different times and this would result in a smear on the gel There is no problem, proteins in the sample would resolve the same as if they were ran on a SDS PAGE gel with a stacking layer on top of a resolving layer QUESTION 18 Sample A is diluted by adding 50 ul to 950 uL of buffer to make solution B. Solution B is diluted by adding 50 uL of solution B to 300 uL of buffer. The absorbance of sample C is 0.05. What is the concentration of sample A assuming the following parameters. |=1 cm Molar extinction coefficient = 10 M-1 cm-1 O 0.7…

Question 8 Which of the following statements is true of eukaryotic transcription? Exons are spliced out from the pre-MRNA. O A 5' cap and 3' poly-A tail are added to protect the mRNA. O Introns are translated into protein. O The protein coding sequence is in the DNA.

Question 11 Carrier proteins are examples of: A.Passive transporters. B.Primary active transporters. C.Entropy-driven pores. D.An ABC transporter. E.Secondary active transporters C « Previous

QUESTION 17 Which of the following best supports the idea that the genetic code is composed of triplet codons? O A. Poly G RNA directs synthesis of poly glycine B. Addition or deletion of one base in a coding region causes an abnormal, defective protein to be produced. O C. Poly U RNA directs synthesis of poly proline. O D. Addition or deletion of three bases in a coding region can result in the production of a protein that stil retains normal function. O E. A and Care both correct.

QUESTION 1 The Wnt signaling pathway component, Dsh, effects the action of B catenin by: O A. inhibiting the function of axin in the destruction complex O B. blocking GSK-3B from joining the destruction complex O C. stimulating ubiquitination of B catenin O D. causing its release into the cytosol from the plasma membrane complex O E. causing an increase in phophorylation of B catenin QUESTION 2 PCR can be used to identify the breakage points of mtDNA that are joined to form a mitochondrial genome with a deletion. This techni que uses: O A. two primers outside the deleted region; a long PCR extension segment. O B. one primer within the deleted region and one primer outside the deleted region; a long PCR extension segment O C. one primer within the deleted region and one primer outside the deleted region; a short PCR extension segment O D. two primers outside the deleted region; a short PCR extension segment O E. two primers within the deleted region; a short PCR extension segment