QUESTION 3 Look at the following piece of DNA cut with enzymes I and II, crating 3 fragments; A, B and C. Which of these is the correct configuration for how they would run on a gel? (remember that DNA is negatively charged and runs towards the positive electrode). II DNA: C 1. O | | B A C 2. O || C 3. O A B 0 1 O 2 O 3

Human Anatomy & Physiology (11th Edition)
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ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
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Chapter1: The Human Body: An Orientation
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**Question 3**

Look at the following piece of DNA cut with enzymes I and II, creating 3 fragments: A, B, and C. Which of these is the correct configuration for how they would run on a gel? (Remember that DNA is negatively charged and runs towards the positive electrode).

**DNA:**

Fragment positions:
- I: A
- II: B
- C

**Configurations:**

1. Configuration 1
   - Position of fragments from negative to positive electrode: B, A, C
   - Electrode orientation: (+) -------- (-)

2. Configuration 2
   - Position of fragments from negative to positive electrode: B, C, A
   - Electrode orientation: (-) -------- (+)

3. Configuration 3
   - Position of fragments from negative to positive electrode: C, A, B
   - Electrode orientation: (+) -------- (-)

**Options:**

- ○ 1
- ○ 2
- ○ 3
Transcribed Image Text:**Question 3** Look at the following piece of DNA cut with enzymes I and II, creating 3 fragments: A, B, and C. Which of these is the correct configuration for how they would run on a gel? (Remember that DNA is negatively charged and runs towards the positive electrode). **DNA:** Fragment positions: - I: A - II: B - C **Configurations:** 1. Configuration 1 - Position of fragments from negative to positive electrode: B, A, C - Electrode orientation: (+) -------- (-) 2. Configuration 2 - Position of fragments from negative to positive electrode: B, C, A - Electrode orientation: (-) -------- (+) 3. Configuration 3 - Position of fragments from negative to positive electrode: C, A, B - Electrode orientation: (+) -------- (-) **Options:** - ○ 1 - ○ 2 - ○ 3
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