QUESTION 1 The relative amounts of each nucleotide base are tabulated below for four different viruses. Virus T CUG A 1 34 42 0 18 39 || III 0 24 35 27 17 0 12 9 12 9 IV 23 16 0 16 23 For each virus, indicate whether its genetic material is DNA vs. RNA and double-stranded vs. single-stranded by putting the correct letter in the box next to each virus. Every choice will be used once and only once. Virus I: Virus II: Virus III: Virus IV: a. Single-stranded DNA b. Double-stranded DNA c. Single-stranded RNA d. Double-stranded RNA

35. M Heinz Shuster collected the following data on the base composition Move ANALYSIS ribgrass mosaic virus (H. Shuster, in The Nucleic Acids: Chemistry and Biology, vol. 3, E. Chargaff and J. N. Davidson, Eds. New York: Academic Press, 1955). On the basis of this information, is the hereditary information of the ribgrass mosaic virus RNA or DNA? Is it likely to be single stranded or double stranded? Percentage A T U Ribgrass 29.3 25.8 18.0 0.0 27.0 mosaic virus Leibniz Institute for Age Research, Fritz Lipmann-Institute. Ribgrass mosaic virus.

Question 13 If a recombinant plasmid (below) was obtained inserting DNA into the BamHI site, screening for the recombinant plasmid can be done by which of the following technique? A) Plate on agar plates containing tetracycline and ampicillin. (B) Plate on agar plates containing ampicillin, C) Plate the cells on agar containing ampicillin then surviving colonies are plated on another agar plates with tetracycline. D) Plate on agar plates containing tetracycline. Pal Pord- ampr EcoRI ПРИ Ano pBR322 (4363 bases) Prull BamHI Sall let" Aval -Sall

Question 1: Look at the following normal and mutant DNA sequences. Normal Sequence (5'-3'): ATG AAC GTT ATC GCA Mutated Sequence (5'-3'): ATG AAT GTC ATC GCA a) What type of mutation has occurred (be specific)? b) Fill in the table for the normal and mutated sequences. Starting with the given 5'-3' sequence, input the complementary DNA, transcribed RNA from the 3'-5' DNA and translated polypeptide sequence for both. Hint: use the codon table! Normal sequence Mutant sequence DNA 5'-3' (given) DNA 3'-5' RNA Polypeptide (use 3 letter codes) c) Based on parts A and B above, what is the ultimate effect this mutation has had on the polypeptide? (1 sentence summary)

Question 2. For your senior research, you end up studying the life cycle of an animal virus whose genome consists of a single circular, double-stranded DNA molecule. Your project is to define the number and location of the origin(s) of replication and to determine whether replication proceeds in one (unidirectional) or both (bidirectional) directions away from an origin. To accomplish this, you isolate many identical strands of DNA that have already been partially replicated. You cleave each piece of DNA exactly once with a restriction enzyme. You then observe the cut pieces of DNA using an electron microscope. Below is a schematic representation of what you observe. Remember that each line represents a different piece of DNA, and not a fragment of a larger piece. Using this data, answer the following questions: A) How many origins of replication do you think the viral genome has, and why do you think this? 30: F2 #3 *** E D 80 F3 54 $ 4 F4 R LL % 5 F5 T G B) Do your data support…

ntrol A option N S X H command C V G B I N M command ? ?? option Name four differences in the structure and location of DNA VS RNA

QUESTION 6: Based on your knowledge of DNA replication, indicate the polarity of each primer.

Question 39 Where would you find the chromosome of a bacterium? bacteria do not have chromosomes plasmid nucleoid nucleus

b) Among the progeny from the Hfr3 mating above, you find one that has the genotype: Abc de F Draw out the gene transfer and crossover(s) that produced this outcome: c) Among the progeny from the Hfr3 mating above, you find one that has the genotype: A B c d e f Draw out the gene transfer and crossover(s) that produced this outcome:

Regular DNA replication Question 3 options: Occurs through the addition of nucleotides to the end of the parental DNA molecule Results in the formation of four new DNA strands Uses one RNA molecule as a template for the creation of a new strand Begins when two DNA molecules join together to exchange segments Not listed