Q: Outline the steps to isolate a typical protein. What isachieved at each step?
A: Protein isolation is a process which involves isolation of proteins from a mixture of compounds.…
Q: What properties are required for a molecule to function as anautoinducer?
A: The molecule that is responsible for the transfer of information between body cells are called…
Q: technique is used to identify the binding partner for Izumo?
A: Sexual reproduction helps in the increase in progeny of almost all eukaryotic organisms. Sperm meets…
Q: Explain about the BLAST (Basic Local Alignment Search Tool) ?
A: The nucleotide is the organic molecule that is the building block of DNA and RNA. They also play…
Q: Why would a a pure protein screening approach be used to discover a small molecule inhibitor of a…
A: INTRODUCTION A small molecule screen is a procedure in which small molecules (typically organic…
Q: What is VNTR profiling, and what are the applications of thistechnique?
A: DNA profiling is a forensic technique in criminal investigations. It is used as a person's DNA to…
Q: Is the ubiquitination relatively stable or highly dynamic? how does ubiquitination become detached…
A: The translation is a process through which ribosomes synthesizes polypeptide chains using mRNA. This…
Q: How do you verify amino acid sequences with proteins and species with BLAST?
A: Bioinformatics is an interdisciplinary field of study which has diverse application in the…
Q: xplain using 2-3 sentences the biological significance of the following peptides. Ceruloplasmin…
A: A peptide bond is generally a covalent chemical bond that conncedting two consecutive amino acid…
Q: What is this quantitative method called?
A: A mass- a spectrometry-based method that is used for quantifying and identifying changes in more…
Q: how UTI's could be prevented ?
A: UTI is urinary tract infection. It is an infection in the urinary system like kidneys, ureters,…
Q: What are the limitations of BLAST (Basic Local Alignment Search Tool)?
A: Introduction Basic local alignment search tool is an algorithm and programme for comparing primary…
Q: What is the 1% extinction value of the protein used to calibrate this experiment? I obtained a…
A: Extinction coefficient It is defined as the absorbance divided by the concentration and the…
Q: How might the technique of affinity chromatography be used to purify lectins ?
A: Lectin is a protein that can recognize carbohydrates and can bind to them. Affinity chromatography…
Q: what is the pros and cons of the protein sythesis in vivo or SPPS? Is in vivo peptide bonding…
A: Inside the cell under invivo conditions the protein synthesis is carried out by complex machinery…
Q: Design a diagnostic test kit based on bio-sensor principles, for COVID-19. (Specify the main…
A: Introduction :- Covid-19 is a very contagious infection ( disease) caused by the SARS-CoV-2. It…
Q: From where Probes are derived ?
A: DNA(deoxyribonucleic acid) is a double-stranded helical genetic material containing thousands of…
Q: Briefly explain in situ hybridization, giving some applications of this technique.
A: Introduction Molecular biology deals with various techniques for study of structure and functioning…
Q: In
A: STR typing or short tendum repeat analysis is a molecular biology technique used to study or analyze…
Q: Define Kinetic Resolution of Amino Acids Using Enzymes ?
A: Kinetic resolution (KR) is a process in which one of the enantiomers of a racemic mixture is…
Q: What is the purpose of Aseptic technique?
A: The living environment is always surrounded by various microbes, the room temperature paves the…
Q: What is the biochemical advantage of sigmoidal kinetcs?
A: Sigmoidal kinetics is the result of the enzyme that shows the positive cooperative binding i.e the…
Q: Briefly describe the Kirby-Bauer test and its purpose.
A: Prokaryotes are the primitive organisms that were the first to develop and harness life on Earth.…
Q: For genotype: repP lach lacPt lacO+ lacZt lacY Select the best description of beta-galactosidase…
A: Beta galactosidase is an enzyme which breaks the disaccharide lactose to produce galactos and…
Q: Explain the importance of the proper targeting of nascent polypeptides
A: Protein targeting is the biological process by which proteins are transported to their destinations.…
Q: Immunofluorescence and Fluorescent live cell imaging techniques can both be used to determine…
A: Immunofluorescence is a technique in which fluorochrome labeled-antibodies are used to detect the…
Q: Briefl y describe the Kirby-Bauer test and its purpose.
A: Antibiotics are antibacterial drugs. Antibiotic drugs are commonly used in the treatment and…
Q: Based on your Lineweaver-Burk plot, what is the mode of PaESBL-1 inhibition by NSAMB?
A: Since you have asked multiple question, we will solve the first question for you. If you want any…
Q: Treated onion root tip with colchicine and untreated onion root tip model. What is the notable…
A: Colchicine is a toxic chemical that is often used to induce polyploidy in plants. Basically, the…
Q: Q: Define electrophoresis. What are the different types of electrophoresis used in separation of…
A: Electrophoresis is a laboratory technique that is performed to separate biological molecules like…
Q: Please answer ASAP: What is Shine-Dalgarno sequence? In which groups of microorganisms it is…
A: The "translation" is the process through which mRNA codes for a certain protein. The ribosome…
Q: What type of probes must be used for super-resolutionmicroscopy and why?
A: The super-resolution microscopy involves a series of techniques that allow high-resolution images.…
Q: Consider the leucine transporter. Would Vmax and/or Kt change if you added a Na+ ionophore to the…
A: As glucose is pumped from the intestine into the epithelial cell at the apical surface, it is…
Q: Identify the role of ubiquitin and describe the enzymes required for ubiquitination
A: Ubiquitin is a small polypeptide that is highly conserved in eukaryotes. There is a high degree of…
Q: What are some common protein identification techniques?
A: Proteome: The set of all expressed proteins in a cell, tissue, or organism. Proteomics: A science…
Q: Can wearing a mask, limiting public spaces capacity, and having travel restrictions be justified…
A: Covid 19 (Coronavirus disease 2019) is an infectious viral disease caused by SARS-CoV-2 (Severe…
Q: What is the Rf value of each amino acid observed?
A: Rf ( retardation factor):- It is defined as the ratio of distance traveled by the centre of a spot (…
Q: Explain the two-dimensional gel electrophoresis (2DGE) ?
A: Based on size and charge, macromolecules & their fragments can be separated and analysed using…
Q: s in protein assays? Explain briefly.
A: Protein assays can be utilized as the methods life science research in order to estimation of…
Q: outline the five main steps used to make a karyotpe?
A: Karyotype is a simple picture of a person's chromosomes. In order to get this picture, a person's…
Q: How can single-cell genomics be used to addressmicrobial dark matter?
A: Microbes have a key role in plant growth, health and they also control certain climates and…
Q: Define a technique for separating hundreds to thousandsof proteins with high resolution ?
A: Introduction: The protein of protein purification involves the separation of the protein and…
Q: . What is the full form of RFLP?
A: Restriction enzymes are the endonucleases that cleave DNA specific locations. random DNA mutations…
Q: What is “scale”? What are the solubilty equilibria involved in scale formation?
A: Scaling deposits are common in pipes and are formed as a consequence of hardness in water.
Q: What are the Normal Values for Fluorescence In Situ Hybridization (FISH) and what can I interpret…
A: Fluorescence in situ hybridization (FISH) is a research facility strategy for distinguishing and…
Q: What phi and psi angles would you predict to be most prevalent for myoglobin and green fluorescent…
A: The 1st globular protein was myoglobin whose structure has worked out from the X-ray diffraction by…
Q: What is probe? Why it is used in the library screening ?
A: Biotechnology is the practice of using living organisms such as animals, plants, microbes, and…
Quantitatively describe the unique focusing capability of IEF and identify factors that increase resolution of proteins in IEF.
IEF stands for isoelectric focusing which is a technique used to resolve a mixture of proteins on a gel. It depends upon the isoelectric points (pI) of different proteins. A combination of IEF with SDS-PAGE (sodium dodecyl sulfate-polyacrylamide gel electrophoresis) known as 2D (dimensional) gel electrophoresis is used widely to perform protein separation.
Step by step
Solved in 2 steps
- . Throughout downstream processing, various analytical methods must be used to evaluate the effectiveness of unit operations. Provide a method or methods (if more than one is required) for each scenario. Quick determination of the concentration of a purified sample of monoclonal antibody. Determine the concentration of monoclonal antibody in clarified extract. Study aggregation of a protein product without disruption of tertiary protein structure. Characterize phenolics (secondary metabolites and impurities produced by green plant tissue) by identifying phenolic species and relative concentrations.Explain how COVID-19 can be detect using molecular basedExplain ORF analysis.
- Define RFLPs and give their significance.Throughout downstream processing, various analytical methods must be used to evaluate the effectiveness of unit operations. Provide a method or methods (if more than one is required) for each scenario. Assess total protein in rice flour (solid material). Visualize antibody fragments (heavy chain and light chain) and compare molecular weights. Quick determination of the concentration of a purified sample of monoclonal antibody. Determine the concentration of monoclonal antibody in clarified extract. Study aggregation of a protein product without disruption of tertiary protein structure. Characterize phenolics (secondary metabolites and impurities produced by green plant tissue) by identifying phenolic species and relative concentrations. Detect protein variants that differ by a substitution of two amino acids. Quantify the level of endotoxin in the final purified product. Quantify bacterial contamination in your final formulated product.pls explain Increasing the saturation of the ammonium sulfate is a prerequisite in isolating a target protein that is rich in Cys and Tyr residues. Which of the following techniques should be considered in accurately quantifying the isolated protein?I. Running the isolated protein in a dialysis or GFC set up.II. Using Biuret or BCA assay as the colorimetric quantitation method.III. Using Bradford or Lowry assay as the colorimetric quantitation method.A. I onlyB. II onlyC. I and IIID. I, II and III. Bradford Assay is most suitable to use when the extraction buffer is below the target protein’s pI. This is so because the protein would be morea. Positively charged allowing the CBB G-250 dye to bind via its sulfonate groups.b. Negatively charged allowing the CBB G-250 dye to bind via its sulfonate groups.c. Neutrally charged allowing the CBB G-250 dye to bind via its sulfonate groups.d. Zwitterionic allowing the CBB G-250 dye to bind via its sulfonate groups.
- How are the Kato Katz Technique results reported?Sketch the appearance after visualization of a protein mixture containing the seven proteins (fibrinogen, y-globulin, collagen, ovalbumin, myoglobin, hemoglobin, insulin) when subjected to two-dimensional (2D) gel electrophoresis.Describe a general scheme for protein purification of a protein like hemoglobin.