How might the technique of affinity chromatography be used to purify lectins ?
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How might the technique of affinity chromatography be used to purify lectins ?
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- What is differential centrifugation? What are its advantages and disadvantages in subcellular fractionation?Suppose you have a cell-lysis sample containing genomic DNA and a complex mixture of proteins and debris. Describe different commonly used analytical methods to i) separate and purify DNA contents from other cellular contents. ii) amplify a gene of interest if present in the genomic DNA.After purifying the expressed protien His6-NEP of interest, the different fractions collected will be run on an SDS-Page gels. If our purification was successful a band of correct size should be present in: A) orginal lysate B) lysate flowthrough C) wash flowthrough D) Elute E) orginal lystae and elite fractions
- Ms. Michi is a researcher in a renowned pharmaceutical company based in Singapore. One of the cell lines in the lab is suspected to be contaminated. She is culturing NIH 3T3 mouse embryo fibroblast cell line (NIH/3T3 (ATCC® CRL-1658TM). Figure 1 below illustrates her observation under the inverted phase contrast microscope. a) b) c) d) e) Figure 1 (Routray et al., 2016) What type of contamination is her laboratory facing? Explain your answer. Give FOUR (4) suggestions to Michi in order to prevent such contamination in future Besides the contamination stated in Question 2 a), name FIVE (5) other contaminations that Michi should be aware of. During a routine check, Michi's superior requests her to characterize cell lines available in the storage to determine their authenticity. Describe TWO (2) effective cell line characterization methods that she can utilize for this purpose. The pharmaceutical company wants to improve its toxicity testing by replacing monolayer culture with spheroid…I understand that microarrays are being used to define the molecular abnormality and the prognosis in some patients with leukaemia. What are microarrays?How can cell fractionation be used to determine cancer cells response to LPS and nanomaterials?
- What is the expansion of acronym MICA?Regarding the cultivation of animal cells in vitro, it is correct to state that: *(Only one statement is correct) a) Transformed cells differ from normal and established cells in that they have the ability to proliferate indefinitely in culture b) Transformed cells derive from isolated and dissected tumors, constituting cell lines that have limited cultivation time c) The genetic instability presented in normal cells makes them preferential models for the production of recombinant proteins d) Cells cultured in adhesion must be periodically expanded, through the use of enzymes such as trypsin e) The metabolism of carbon sources in the culture medium by animal cells generates products such as lactate, which positively influence their productivitydescribe how lysozyme may or may not be separated by thin-layer chromatography
- What is differential centrifugation? Explain how it can be used to separate cellular organelles.Immunofluorescence and Fluorescent livecell imaging techniques can both be used to determine protein localisation.List the advantages and disadvantages of using immunofluorescence andfluorescent live cell imagingDuring IHC staining, where would alpha-tubulin show in the esophagus image below (see image)?