Q: When would it be beneficial to use a eukaryotic host formolecular cloning?
A: The techniques for gene manipulation, cloning, and expression were first developed in bacteria.…
Q: Remember that although there are many interesting ideas about genetic engineering of plants and…
A: Genetic engineering is a one of the modern technology to modify organisms, plants, crops & even…
Q: Explain the Retroviral vectors of Gene Therapy ?
A: Retrovirus is used to infect the mammalian cells as they used host cells to multiply. Retrovirus is…
Q: Is natural bacterial transformation efficient?
A: Bacterial transformation is the process by which a bacterium takes up naked DNA from the…
Q: What would happen if recombinant microbes could transfer genetically altered genes into other…
A: This is a major issue that's why there are so many methods are invented for the biotechnology…
Q: What is the difference between genetic horizontal gene transfers: transformation, transduction,…
A: Horizontal gene transfer is the movement of genetic information between unicellular and…
Q: do you think, CRISPR-cas9 a panacea for genetic engineering, justify your answer?
A: Answer: GENETIC ENGINEERING = It is the molecular process of using recombinant DNA (rDNA)…
Q: How did clone USA300 and other strains of MRSA become so dangerous?
A: The reason is;
Q: Which of the following viruses have been used as vectors for gene delivery?
A: Gene delivery is a process of introducing the foreign genetic materials which are DNA and RNA into…
Q: How is Ti plasmid of Agrobacterium tumefaciens modified to convert it into a cloning vector?
A: Agrobacterium tumefaciens is a well-studied species of the gram-negative genus of bacteria, known as…
Q: How did Anton de Bary prove that a microbe was the cause of the Irish Potato Famine? Why did other…
A: Irish potato famine is a type of infection found on potato which is otherwise called a potato blight…
Q: 1.Discuss how PCR may be used for the detection of disease-causing pathogens in a population during…
A: We are answering 1st question only. For the rest of the questions please repost. Because of the…
Q: n example of bioremediation is A) the use of prokaryotes to treat sewage or clean up oil…
A: BIOREMEDIATION:- It has been defined as a breakability technology that uses biological activity to…
Q: What modification is done on the Ti plasmid of Agrobacterium tumefaciens to convert it into a…
A: Recombinant DNA technology (rDNA technology) is very helpful for analysing the entire genome of a…
Q: Why are the recombinantDNA technology and thenucleus transplantationtechnology still dangerous?
A: Recombinant DNA technology basically refers to the joining of DNA molecules from two different…
Q: Why doesn't the CRISPR system cut the bacterium's own DNA, at the point in its genome where it…
A: CRISPR is an acronym for Clustered Regularly Interspaced Short Palindromic Repeat. This name refers…
Q: What is the purpose of using DNA polymerase from Thermus aquaticus for the polymerase chain reaction…
A: Thermus aquaticus is a heat tolerant bacterium .DNA polymerase used from Thermus aquaticus is heat…
Q: When a bacteriophage accidentally incorporates a fragment of host DNA in its capsid and delivers it…
A: Bacteriophages bind to various receptors on the surface of bacteria, such as lipopolysaccharides,…
Q: How has the CRISPR editing technology been appliedto targeting virus-infected eukaryotic cells?
A: The short form CRISPR refers to the Clustered Regularly Interspaced Short Palindromic Sequences.…
Q: Why do bacteria that are not genetically resistant to antibiotics die out when exposed to…
A: Bacteria are prokaryotic organisms that have an undefined nucleus and nuclear membrane. Most of the…
Q: In the phage titer experiment, why did you plate multiple dilutions? a. so that you should have a…
A: Bacteriophage It is a type of virus that infect bacteria.
Q: Should the DNA sequence encoding human pathogens be freely available online? and Why?
A: DNA sequencing is technique which helps in identification of exact sequence of bases (A,C,G and T)…
Q: How are inactivated vaccine and mRNA vaccine produced? From what you know about DNA technology and…
A: Inactivated vaccines are made up of virus particles, bacteria, or other pathogens that have been…
Q: Create recombinant DNA molecules with plasmid vectors?
A: The DNA (deoxyribonucleic acid) is the genetic material of an organism that is inherited by the…
Q: Identify one specific example of how this process has been used 10. Using the example provided in…
A: Transgenic organisms are created by manipulating genes in the the plant of the animal species. Thud…
Q: If HIV was just an enveloped virus, but not a retrovirus, which of the following drugs would likely…
A: Virus captures the host replication machinery and multiplies its genome. This is the foundation of…
Q: Give any two differences between DNA and RNA. (b) What is recombinant DNA? Give two beneficial…
A: Differences between DNA and RNA DNA RAN Double stranded Single stranded Consists of…
Q: Do you see any potential problems with inserting pieces of a retroviral genome into humans? If so,…
A: Viruses are a nucleoprotein entity which is able to utilize the synthetic machinery of a living cell…
Q: What is the major difference between genomic DNA and cDNA? List various vectors and state which is…
A: The main difference between genomic DNA and cDNA is cDNA: cDNA is synthetic dna base pairs are…
Q: What is transgenic food?
A: Recombinant DNA technology involves integrating DNA molecules from two different species and…
Q: Give three reasons why Salmonella strains are very useful for mutagenesis studies
A: Ans- The standard set of bacterial strains that are used in the mutagenicity testing of…
Q: Review the Process Diagram, Figure 8.9, and answer this question. The type of horizontal gene…
A: Horizontal gene transfer (HGT) or lateral gene transfer is the movement of genetic material between…
Q: Explain the THREE (3) important regions of this plasmid that enable it to work efficiently as a…
A: Plasmid is a self replicating generally circular DNA molecule that can be used to carry a foreign…
Q: Name any one disease for which gene therapy has been proved effective?
A: Gene therapy is one of the treatment methods to treat genetic diseases. It involves the replacement…
Q: In the bacterial transformation experiment, what is the primary purpose of using an…
A: Transformation experiment was given by Griffith. In bacteria, DNA can be transferred using…
Q: GMOs are created by ________. a. generating genomic DNA fragments with restriction endonucleases b.…
A: The term genetic engineering is used to modify an organism by affecting its genetic constituents. It…
Q: Given that antibiotic-resistant strains of bacteria are a concern to the general health of a…
A: The antibiotics are a special substances that are released by some bacteria to kill or inhibit the…
Q: Based on the image below of a Kirby-Bauer assay, this bacteria is most susceptible to which…
A: Antibiotics are substances that have the ability to kill bacteria or restrict their growth. They are…
Q: What is the value of antibiotics to public health, what is the harm to public health of antibiotic…
A: Antibiotics are also called antibacterial or antimicrobial drugs. They are used in the treatment and…
Q: Which technology facilitates the production of novel DNA molecules by combining sequences from DNA…
A: Introduction: Recombinant DNA technology uses gene cloning and gene transfer to isolate and…
Q: Which PCR variants are used for the accurate diagnosis of virus and how virus quantification is…
A: Introduction: PCR or polymerase chain reaction is a laboratory method used to amplify the genetic…
Q: Uses of PCR and GMOs Discuss how PCR can be used in a population to identify disease-causing…
A: Introduction Biotechnology is the field of Biology where living systems are merged with a bit…
Q: Results from a Kirby Bauer antibiotic assay on a Gram-negative bacterial culture are described as…
A: Intrinsic resistance can be described as "it is a natural resistance property, which is derived…
Q: Humans perfected the use of recombinant DNA technology to produce human insulin from bacteria.…
A: Biotechnology is the branch of Biology which deals with the manipulation of organisms and their…
Q: In the bacterial transformation experiment, what is the primary purpose of using an…
A: Bacteria are Prokaryotic microbes which undergo splitting process and form similar cells. Genetic…
Q: Explain Gene therapy with viral vectors?
A: Gene therapy is a medical technique that is used in the treatment of diseases by the help of genes.…
Q8) Using the pCR 2.1 vector, what antibiotics would a cell with the vector be resistant to?
Q9) What does B-galactosidase do?
Trending now
This is a popular solution!
Step by step
Solved in 2 steps
- Q7) What are things that are common in cloning vectors? Why are they useful?Q8) Using the pCR 2.1 vector, what antibiotics would a cell with the vector be resistant to?Q9) What does B-galactosidase do?20 16. Which of the following methods may introduce foreign DNA into a recipient? A) transduction B) conjugation C) transformation D) All of the above 17. F+ strains of Escherichia coli A) have an integrated F factor. B) have the F factor as a plasmid. C) do not have an F factor. D) transfer the F factor to recipient cells at a high frequency. 18. What is the function of the conjugation pilus? A) It contains an F plasmid. B) It carries the chromosome of F- cells. C) It converts F- cells into F+ cells. D) It pulls the F+ and F- cells together. 19. When a transposon is cut from one location in genome and pasted into a new location, it is called replicative transposition A) True B) False (0 20. Insertion sequences are bounded by inverted repeats and encode for transposase. A) True B) False 21. The CRISPR system UM (8 A) recognizes foreign DNA sequences that have previously entered the cell and directs the Cas proteins to destroy them. B) synthesizes gene transfer agents during stationary…17. Which pair of scientists discovered the structure of the DNA double helix and helped to make recombinant DNA technology possible? 18. In this experiment, which of the following DNA molecules carries the gene for kanamycin resistance? A) the TN903 transposon B) the pUC plasmid 19. In this experiment, which of the following DNA molecules carries the gene for ampicillin resistance? A) the TN903 transposon B) the pUC plasmid 20. In this experiment, both the transposon DNA and the plasmid DNA are cut with which of the following restriction enzymes? А) НаеШ В) ЕcoRI C) SacI D) PvuII 21. The restriction enzyme used to cut the DNA generates: A) blunt ends B) sticky ends 22. In this experiment, what is required to join the transposon fragment with the linearized plasmid? A) the enzyme DNA ligase B) a buffer solution containing ATP C) an incubator set within the temperature range of 4-22°C D) all of the above 23. What is the purpose of treating the linearized plasmid with the enzyme alkaline…
- 1) Follow the steps involved in DNA or gene cloning 2) In what process do bacteria take up the recombinant plasmid DNA 3) What are the advantages or replications of gene cloning?22.Identify the single most correct answer: (a) Ames assay (test) can be used to identify carcinogens but not mutagens. b) Conjugation in viruses can result in production of an antibiotic resistant virus. c) Certain groups of viruses are responsible for the process of transduction in bacteria. d) Organisms in their natural environment cannot exchange genetic information. e) c and d 24. In a use-dilution test, for microorganism X, we evaluated 3 different disinfectants T1, T2 and T3 with the following disinfectant dilutions: 1/10, 1/100, 1/1,000 and 1/10,000. T1 killed the microorganism up to the dilution of 1/100; T2 killed the microorganisms up to the dilution of 1/10,000 and T3 killed the microorganisms up to the dilution of 1/1,000. Rank the disinfectants in the order of their strength with the strongest being first, for disinfecting contaminations with microorganism Xor alternatively are they all equally effective? Choose the most correct answer. a) T1 (the strongest), T2, T3…Q11) Explain, using the pCR 2.1 vector, what we would see if we try to grow cells on a plate with ampicillin and Xgal in each of these scenarios:a) transformation not successful (meaning the plasmid did not go inside the bacteria)b) transformation was successful, but no insert in LacZ gene (plasmid went in the bacteria but there was no inserted DNA in the LacZ gene of the plasmid)c) transformation and the ligation reactions were successful (got the plasmid in, and the plasmid is carrying extra DNA in the LacZ region, yay)
- 1. What is a plasmid? A) An organelle found in plants that conducts photosynthesis. B) Circular extrachromosomal DNA. C) An essential part of the bacterial chromosome. 2. What is the role of the NaOH in plasmid isolation? A) It increases the pH. B) It denatures the DNA. C) Both of the above. 3. In which direction will the DNA migrate in an agarose gel? A) To the positive pole. B) To the negative pole. C) That depends on the sequence of the DNA. 4. What is the function of SDS? A) It denatures proteins. B) It disintegrates the cell membrane. C) Both of the above. 5. To which species group does E. coli belong to? A) Bacteria, B) Fungi. C) Archaea.Results from a Kirby Bauer antibiotic assay on a Gram-negative bacterial culture are described as follows: A) the bacterium is resistant to penicillin, an antibiotic that targets synthesis of the peptidoglycan cell wall and B) the bacterium is resistant to tetracycline, an antibiotic that targets the small subunit of the ribosome, inhibiting protein synthesis. Which of the results represents intrinsic resistant and which represents acquired resistants?Which method is used to obtain mutants that grow under conditions that the wild type parent cannot grow? a)indirect selction b) direct selection c) screening for possible mutagen ( carcinogens) d) replica plating
- 8. A mutant that has a nutritional requirement for growth is an example of a(n) A) heterotroph B) auxotroph C) autotroph D) organotroph 9. In the Ames test the number of histidine auxotroph colonies growing on media lacking histidine when exposed to a potential chemical mutagen is. proportional to the likelihood that the chemical is mutagenic. A) Directly B) Inversely 10. Thymine dimers result from A) ionizing radiation B) nucleoside analogs C) non-ionizing radiation D) none of the above 12. RecA proteins are NOT required for homologous recombination? A) True B) False 13. Increasing genetic variability by the uptake of DNA from the environment is A) transduction B) transformation C) a mutation D) conjugation 14. Which of the following features are common to transformation, transduction, and conjugation? (1) unidirectional transfer of genes (2) incomplete gene transfer (3) homologous recombination (4) meiosis occurring in the recipient A) 1, 2, 4 B) 1, 2, 3 C) 3,4 D) 1,2 15. What is…Which is used for cloning eukaryotic genes but not prokaryotic genes?a) Restriction enzymesb) DNA ligasec) Reverse transcriptased) Vectore) Selectable marker17) What gives Aspergillus sydowii colonies their hairy appearance? a) Hyphae () b) Stipes c) Growing media ) d) Ascocarpoda Jack has designed one primer which is complementary to the DNA of E. coli. He then used that primer for a PCR reaction using DNA isolated from a mixture of bacteria. He got no PC reaction product and hence concluded that there is absolutely no E. coli in the bacteria mixture. His conclusion is .... () True ) False