Q15: A research team interested in mapping human genes discovered a new restriction length polymorphism (RFLP). The nature of the polymorphism is shown in the figure below: RESEARCH METHOD GAATTC CTTAAG GACTTC CTCAAG GAGTTC CICAAG Long fragments Short fragments Туре 1 homozygote Туре 2 homozygote Heterozygote S a. First, they test the RFLP in a family with the pedigree below. The individuals colored blue are affected by hemophelia, a blood clotting disease. Dad Mom daughter1 daughter2 son1 son1 b. Next, they genotyped (determined the RFLP polymorphism, type 1 or type 2) of daughter1's family with the results shown below: husband daughter1 T||

In your opinion, is antibiotic use at farm sites promote the development and spread of antibiotic resistant genes in bacteria? Why or why not please explain based on the article

Three mutations were obtained in a bacterial gene. An antibody is available for the protein product of this gene. Both Northern analysis (RNA separated by electrophoresis, blotted, and probed with DNA) and Western analysis (proteins separated by electrophoresis, blotted, and probed with antibodies) were performed on the mutants. The results are summarized below. Northern Size Western Size 1 2 3 1 2 3 + Long Short Short Long For each mutation, what kind of mutation occurred and how do you know? a) Mutant 1 b) Mutant 2 c) Mutant 3

Choose from : insertion with frameshit, deletion with frameshift, silent mutation, deletion without frameshift, missense mutation, insertion without frameshift, nonsense mutation  The wildtype sequence of a gene is the following: wt:          3' TAC AAA TCT AGC CCG 5'  and the following 3 mutations were found:, indicate what type of mutation this is 1:            3' TAC AAA TCA AGC CCG 5'    ; 2.            3' TAC AAA TCT ATC CCG 5'.     ;  3.            3' TAC AAA AGC CCG 5'.           ;

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Can DNA recombinantion be used to insert human genes into human cells; if so, what are the advanatages of inserting the human gene directly into human cells?

What is an STS? How are STSs generated experimentally? What are the uses of STSs? Explain how a microsatellite can be a polymorphic STS.

E17. Gene mutagenesis is also used to explore the structure and function of proteins. For example, changes can be made to the coding sequence of a gene to determine how alterations in the amino acid sequence affect the function of a protein. Let's suppose that you are interested in the functional importance of a particular glutamic acid (an amino acid) within a protein you are studying. By site-directed mutagenesis, you make mutant proteins in which this glutamic acid codon has been changed to other codons. You then test the encoded mutant proteins for functionality. The results are as follows: Functionality (%) Normal protein 100 Mutant proteins containing Тугosine Phenylalanine 3 Aspartic acid 94 Glycine From these results, what would you conclude about the functional significance of this glutamic acid within the protein?