Q. Write down the importance of post transcriptional modifications? Write in 5 to 6 points with least plagirism in ur own words
Q: 20. Explain the effect of the mutation that occurs among northern European people on LCT gene…
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Q: Review after transcription modification. Match the term and its description. Each term can only be…
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Q: 2) Briefly describe 6 different ways that eukaryotic cells typically regulate Gene Expression
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Q: Q.1. Enumerate the post-transcriptional modifications in a eukaryotic mRNA.
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Q: 4. a) Explain what is meant by the term "alternative splicing" b) what does it help to explain?
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Q: 1. What happens during transcription? Possible sentence frame: Transcription is the process in which…
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Q: How expression systems can be developed to purify the protein of interest?
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Q: What are post transcriptional modifications? Write down their importance Write in detail in ur own…
A: In eukaryotes, the transcription of DNA occurs in the nucleus of the cell, which yields the primary…
Q: Outline how alternative splicing occurs, and describe its benefits.
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Q: 4. Compare the control of gene regulation in eukaryotes and prokaryotes in terms of: a.…
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Q: xplain how ncRNA uses complementary base pairing with DNA to affect gene expression.
A: Central Dogma= DNA->RNA->Protein A non-coding RNA or an ncRNA is a normal functioning RNA…
Q. Write down the importance of post transcriptional modifications?
Write in 5 to 6 points with least plagirism in ur own words
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- Q. Deletion of a single AT base pair from codon number 4 can cause a frameshift mutation in a protein-coding gene. Which of the following additional mutations will restore the reading frame back to “normal” such that the original stop codon will still function? (Note that the amino acid sequence will not necessarily be restored back to normal). Adding a base pair into each of the next two codons. Adding a GC base pair back in where the AT pair was deleted. Adding one base to the next codon and deleting one base from the one after that. Deleting a base pair from each of the next two codons. A. 1,2 and 3 B. 1 and 3 C. 2 and 4 D. 4 only E. All of 1,2,3 and 4 are correctDetails explanation about write a short description about post transcriptional modifications ?. What is an enhanceosome? Why could a mutation in anyone of the enhanceosome proteins severely reduce thetranscription rate?
- MATCH THE FF. WITH THE CHOICES BELOW group of genes one long mRNA with complementary codes of 3 or more genes controlled by negative regulation a tetrameric protein, is the lacI gene product It is a palindromic DNA segment at the upstream region of lac gene has a DNA-binding domain on N-terminus; the C-terminus binds inducer, forms tetramer. an accessory protein to activate transcription regulated both positively and negatively by AraC regulatory elements of arabinose operon It is Regulated Through a Co-Repressor-Mediated Negative Control Circuit an example of autogenous regulation has attenuation regulation mechanism CHOICES: -lac operator -Catabolite activator protein -araBAD Operon -polycistronic mRNA -trp Operon -lac operon -lac repressor -Operon -AraC gene. Outline an experimental approach to determining the average chain growth rate for transcription in vivo. Chain growth rate is the number of nucleotides polymerized per minute per RNA chain.. Outline an experimental approach to determining the average chain growth rate for transcription in vivo. Chain growth rate is the num- ber of nucleotides polymerized per minute per RNA chain.
- For each of the ff. scenario, state whether the gene is up- or down-regulated and briefly explain the reason behind. 5’-------enhancer------insulator-----gene of interest----3’What are post transcriptional modifications? Write down their importance Write in detail in ur own words with least plagirism and give points for importanceState whether the gene is up- or down-regulated and briefly explain the reason behind. DNA lacks methylated cytosine residues.
- Q8. Why do you think it takes three lines to display the amino acid information? Hint: remember that a codon is specified by three bases, e.g., CCG = Proline (circled in Figure 12)..In what ways can insertion of a transposon affect the expression of genes in the neighborhood of the insertion site?I. The retinoic acid receptor (RAR) is a transcription factor that is similar to steroid hormone receptors. Thesubstance (ligand) that binds to this receptor is retinoicacid. One of the genes whose transcription is activatedby retinoic acid binding to the receptor is myoD. Thediagram that follows shows a schematic view of theRAR proteins produced by genes into which one oftwo different 12-base double-stranded oligonucleotides had been inserted in the ORF. The insertion site(a–m) associated with each mutant protein is indicatedwith the appropriate letter on the polypeptide map.For constructs encoding proteins a–e, oligonucleotide 1(5′ TTAATTAATTAA 3′ read off either strand) wasinserted into the RAR gene. For constructs encoding proteins f–m, oligonucleotide 2 (5′ CCGGCCGGCCGG 3′)was inserted into the gene.NH2 f g h i j k l m COOHa b c d eThe wild-type RAR protein can both bind DNA and activate transcription weakly in the absence of retinoic acid(RA) and strongly in RA’s presence. Each…