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- Why is it important to limit the quantity of cells used to prepare a smear? Mark all that apply: O So that cells are not clumped and don't entrap stain creating erroneus results So that no contaminants are introduced onto the slide by being entrapped in clumps OSo that the cells are spread out enough that cell morphology can be discerned OSo that the cells are spread out enough that the arrangement can be observed O So that there are small groups of cells clumped together to make them visible Microsoft Bing 11:31 AM 87°F Sunny 9/14/2021What is the purpose of fixing a smear? Mark all that apply: 1. To attach the bacteria to the slide 2. To cause the cells to shrink and become distorted 3. To kill the bacteria so they aren't harmed by the staining method 4. To break down the cell wall in order to make the cells accept stain 5. To kill the bacteria to make the slide safer to handleThe benefits of the negative stain include: Mark all that apply: O No shrinkage or distortion of cells due to no heat or methanol fixing Because the cells don't take up stain they don't get shrunk or distorted O One can more accurately determine cell size and shape because the cells stand out against the background One can more accurately determine the cell size and shape because there is no shrinkage or distortion of cells O Negatively charged dyes are safer to work with than positively charged dyes IMicrosoft B 11:32 A
- I Why does't a negative stain colorize the cells in the smear?Why is it important to limit the quantity of cells used to prepare a smear? Mark all that apply: 1. So that cells are not clumped and don't entrap stain creating erroneous results 2. So that the cells are spread out enough that cell morphology can be discerned 3. So that there are small groups of cells clumped together to make them visible 4. So that no contaminants are introduced onto the slide by being entrapped in clumps 5. So that the cells are spread out enough that the arrangement can be observedIt is called a "Negative Stain" because the stain has a negative charge, and is therefore: repelled by the bacterial surface attracted by the bacterial surface able to penetrate the bacterial surface
- Bacterial smears are fixed before staining to O make their walls permeable. accept stain, affix the cells to the slide. O make the cells visible.A capsule stain is kind of: A Simple and Negative Stain B Differential and negative stain C Simple Stain D Differential stain E Negative StainHow does smear preparation of cells from a liquid medium differ from preparation of cells from a solid medium? Mark all that apply: O Water must be added to a smear from liquid media but not from solid O Water must be added to a smear made from solid media but not liquid media A smear made from liquid media will be more thick than a smear made from solid O A smear made from liquid media will be thinner than a smear made from solid media O A smear made from liquid media doesn't have to be fixed but a smear from solid media does Microsoft Bing 11:31 AM 87°F Sunny 9/14/2021
- Which one of these techniques does not distinguish between live and dead cells? Group of answer choices Coulter count fluorescence staining spread plateSpecific Dyes used in flagella stain: A wet-mount procedure (Ryu method) Dried-smear preparation (Leifson staining technique)In order to do electron microscopy the samples had to be specially prepared. Were the cells alive at the time of viewing? Explain why you said yes or no I need help answering this queshtion the answer is with the article and it has to be as short as possible URL: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC106848/