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- Discuss how DNA extraction of microbial DNA directly can be done from infected tissue through an optimized protocol for use in nanopore sequencing.Comprehensively discuss the principle and applications of PCR. Discuss the quality criteria for nucleic acid isolates. Explain the use of UV spectrometry in nucleic acid purification.List four reasons for the development of pulsed field gel electrophoresis
- At what stage of the culture should bacterial colonies be harvested for plasmid DNA extraction? How about for genomic DNA extraction? What distinguishes the xanthogenate-based methodology from the traditional phenol/chloroform method for isolating DNA from bacteria? What makes potassium ethyl xanthogenate efficient in isolating DNA from a variety of microorganisms?Among various dye-based assays like Biuret, Lowry, Bicinchronic Acid, and Biuret Protein Assays, what can be done if a concentrated sample is out of the detection range of chosen dye-based assay?Briefly describe the principle and the procedure of organic (Phenol-Chloroform) DNA extraction method. Mention its pros and cons.