pH with 0 mL of pH with 5 mL of pH with 10 mL of Cola pH with 20 mL of Cola Buffer Cola Cola Buffer F 4.5 4.8 4.1 4.4 Buffer H (water) 7.0 3.2 n/a n/a Is Buffer F (the acetic acid - sodium acetate buffer you made) a good buffering system for acids? Explain your reasoning.
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- Calculate the mole ratio of sodium acetate and acetic acid needed to make a buffer of p H 4.68 ( see Table 15.1 for Ka values or Appendices G and H for Ka and Ksp values respectively.Calculate the volume of TAE buffer that you will need to prepare a 100ml solution of 1X strength if you only have a 50X concentrated solution available.A buffer contains 0.015 mol of lactic acid (pK₁ = 3.86) and 0.080 mol of sodium lactate per liter. H₂C OH Lactic acid OH Calculate the pH of the buffer. H₂C. Calculate the change in pH after adding 9.0 mL of 0.10 M HCl to 1.0 L of the buffer. O OH Sodium lactate Calculate the change in pH after adding 9.0 mL of 0.10 M HCl to 1.0 L of pure water. O Na+ buffer pH: buffer pH change: water pH change: units units
- Using the Henderson-Hasselbalch equation, calculate the pH of a buffer solution made from 0.20 M CH3COOH and 0.050 M CH3COO- that has pk3= 4.7. 4.1 0.4 None of the answers is correct 2.5 5.3At what pH does a lysine solution exhibit the highest buffering capacity? (Lysine pKas: pk1 = 2.2, pK2 = 8.95, pK3 = 10.5) %3D O рH 12.1 О рH 9.72 O pH 5.67 pH 8.95If 4 mL of 1 M NaOH is added to 100 mL buffer, would it still be a usable buffer according to the conventions? Explain why or why not.
- From the Henderson-Hasselbach equation, calculate the ratio of dihydrogen phosphate (H2PO4-) and monohydrogen phosphate (HPO4-2) components required to produce buffer solution with1. pH 6.22. pH 7.23. pH 8.2. An acetate/acetic acid (pKa=4.75) buffer of 500 mL includes 0.1 mol of acetate and 0.5 mol of acetic acid. 2.0 M NaOH was added to the buffer to get the pH to 4.70. What volume of NaOH was added to the system?determine the pKa of the acid and ph of the buffer sol'n of a buffer mixed with 0.1 M tris and 0.5 M chloine salt having dissociation constant of 1.072x10^-6.
- Calculate pH of buffer prepared by mixing; 10ml of 0.10M of CH3COOH and 20ml of 0.10M of CH3COONa.How much of the enzyme proteinase k (solute) is required to make 250ml of a solution with a concentration of .01mg/ml? (Weight/volume ratio)Calculate pNF concentration in each cuvette using Beer-Lambert’s Law. ε = 18,000 M-1 cm-1; b = 2.00 cm Cuvette 1 pNF 1 mL Water 2 mL Absorbance 0.546