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1. Given the following words (Pentose Phosphate pathway,
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- 1. Given the following words (Glycolysis, ATP yield, Oxidative phosphorylation), make a short explanation on how the given words connect to each other using the concepts in microbial metabolism. Answer this comprehensively.1. Given the following words (Cytoplasmic membrane, ATP synthase, chemiosmosis), make a short explanation on how the given words connect to each other using the concepts in microbial metabolism. Answer this comprehensively.1. Given the following words (Anaerobic respiration, Fermentation, Aerobic respiration), make a short explanation on how the given words connect to each other using the concepts in microbial metabolism. Answer this comprehensively.
- 1. Given the following words (Fermentation, cheese, substrate level phosphorylation), make a short explanation on how the given words connect to each other using the concepts in microbial metabolism. Answer this comprehensively.Which of the following experimental approaches is likely must complete picture of a particular cell's metabolism? a. NMR spectroscopey b. Metabolic inhibitors c. Mass spectroscopy d. Isotopic tracers5)A certain aerobic organism is able to metabolize the followingglycolipid CH,OH H H OH HO OH
- List metabolic pathways of relevance in industrial microbiology and biotechnology Comment on the importance of knowledge of metabolic pathways on production of various microbial products 3. Distinguish between products of primary metabolism and products of secondary metabolism and explain why this classification is important in product recoveryWITH REFERENCES DONT COPY PASTEProvide an example of an organism within each of the four main nutritional categories of microbes and describe how each obtains its essential nutrients. Provide evidence in support of or refuting the following statement: Microbial life can exist in the complete absence of both sunlight or organic nutrients. Polymerase chain reaction (PCR) is a technology that requires high temperatures to reproduce DNA fragments. Explain why the discovery of thermophilic archaea and their associated DNA polymerases was critical to the success of this technique. Summarize how the electron transport chain functions to produce ATP in a bacterial cell versus eukaryotic cell. Explain whether or not the term chemiosmosis accurately illustrates this process.Picture. 1: Fresh potato +H202 H2O+02 (air bubbles) Questions: 1. Why did you use buffer instead of distilled water to dilute the enzyme and the substrate? 2. What do we mean by enzyme specificity? 3. Name the substrate of peptidase, sucrase and amylase? 4. Discuss the factors affecting the rate of an enzymatic reaction, showing how each one affects the rate? 34
- 1. You set up two cultures with an equal amounts of Escherichia coli. You label one culture "A" and the other "B." Each culture is given 100mL of tryptic soy nutrient broth. You then take culture A and incubate it in the absence of oxygen and take culture B and incubate it in the presence of oxygen for one week. If E. coli is able to use both aerobic and anaerobic metabolism, the how would we classify it? Is it an obligate aerobe, obligate anaerobe, or a facultative anaerobe? Based on that information, which flask, A or B, would have more cells at the end of the week? Fully explain your answer.1. Which of the following organisms would you use to change alkaline conditions to acidic conditions? a. B. cereus-VP + b. S. aureus- Glucose metabolism test + c. E. coli- Decarboxylase + d. P. vulgaris- Simmon's citrate + e. E. aerogenes- Gelatinase + 2. Which of the following results is similar to your carbohydrate metabolism tests results when bacterial metabolic byproducts initiated a color change of red to yellow in the agar? a. B. cereus-MR + b. S. aureus-VP+ c. E. coli- Decarboxylase + d. P. vulgaris- Simmon's citrate + e. E. aerogenes- Gelatinase + 3. After an acid-fast stain, a gram positive organism would be a. blue b. green c. cranberry red d. yellow e. orange 4. Your patient is on a diet where he consumes a lot of protein. Due to this diet and the over consumption of proteins, he is dangerously close to his blood becoming acidic. So therefore, which of the following did you administer (hypothetically) to buffer the acids? a. B. cereus: VP b. S. aureus: Decarboxylase + c. L.…What are the general concepts of microbial metabolism and explain each concepts? (Give at least 4 concepts)