Observation 1. potato extract + phenol + H,02 2. potato extract + catechol + + H,0, 3. potato extract + Guiac + H202 1. boiled potato extract + phenol + H202 2. boiled potato extract + catechol + + H,O2 3. boiled potato ex tract + Guiac + H,0,2

Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
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Laboratory Report Sheet – ENZYMES

Procedure:

Potato Peroxidase

  1. a. Prepare 3 test tubes. Place 1 mL of potato extract  to each test tubes.

To the first test-tube  -   add 5 drops of 1% phenol+ 3 drops of H2O2

        second test tube –  add 5 drops of catechol  or pyrogallol + 3 drops H2O2

        third test tube -   add 5 drops of guiac solution +

                3 drops H2O2

b. Shake well and observe change in color.

2. Repeat procedure 1, a, b, and c, but this time use boiled potato extract.

QUESTION: WHAT ARE THE OBSERVATIONS FOR EACH TUBE?

I. DATA & RESULTS
C. Potato Peroxidase
Observations
1. potato extract + phenol + H,02
2. potato extract + catechol + + H,02
3. potato extract + Guiac + H202
1. boiled potato extract + phenol + H202
2. boiled potato extract + catechol + + H,O,
3. boiled potato ex tract + Guiac + H,02
Transcribed Image Text:I. DATA & RESULTS C. Potato Peroxidase Observations 1. potato extract + phenol + H,02 2. potato extract + catechol + + H,02 3. potato extract + Guiac + H202 1. boiled potato extract + phenol + H202 2. boiled potato extract + catechol + + H,O, 3. boiled potato ex tract + Guiac + H,02
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